Scopus
YÖKSİS Eşleşti
Proteomic fertility markers in ram sperm
Animal Reproduction Science · Aralık 2021
YÖKSİS Kayıtları
Proteomic fertility markers in ram sperm
Animal Reproduction Science · 2021 SCI-Expanded
PROFESÖR MUSTAFA NUMAN BUCAK →
Proteomic fertility markers in ram sperm
Animal Reproduction Science · 2021 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiAnimal Reproduction Science
Yayın TarihiAralık 2021
Cilt / Sayfa235
Scopus ID2-s2.0-85119405404
Özet
Precise estimation of ram fertility is important for sheep farming to sustain reproduction efficiency and profitability of production. There, however, is no conventional method to accurately predict ram fertility. The objective of this study, therefore, was to ascertain proteomic profiles of ram sperm having contrasting fertility phenotypes. Mature rams (n = 66) having greater pregnancy rates than average (89.4 ± 7.2%) were assigned into relatively-greater fertility (GF; n = 31; 94.5 ± 2.8%) whereas those with less-than-average pregnancy rates were assigned into a lesser-fertility (LF; n = 25; 83.1 ± 5.73%; P = 0.028) group. Sperm samples from the outlier greatest- and least-fertility rams (n = 6, pregnancy rate; 98.4 ± 1.8% and 76.1 ± 3.9%) were used for proteomics assessments utilizing Label-free LC-MS/MS. A total of 997 proteins were identified, and among these, 840 were shared by both groups, and 57 and 93 were unique to GF and LF, respectively. Furthermore, 190 differentially abundant proteins were identified; the abundance of 124 was larger in GF while 66 was larger in LF rams. The GF ram sperm had 79 GO/pathway terms in ten major biological networks while there were 47 GO/pathway terms in six biological networks in sperm of LF rams. Accordingly, differential abundances of sperm proteins between sperm of GF and LF rams were indicative of functional implications of sperm proteome on male fertility. The results of this study emphasize there are potential protein markers for evaluation of semen quality and estimation of ram sperm fertilizing capacity.
Yazarlar (12)
1
Mustafa Hitit
2
Mehmet Özbek
3
Serife Ayaz-Guner
4
Huseyin Guner
5
Merve Oztug
6
Mustafa Bodu
ORCID: 0000-0002-8432-7347
7
Mesut Kirbaş
8
Bülent Bülbül
9
Mustafa Numan Bucak
10
M. B. Ataman
11
Erdoğan Memili
12
Abdullah Kaya
Anahtar Kelimeler
Fertility
Proteomics
Ram
Sperm
Kurumlar
Abdullah Gül Üniversitesi
Kayseri Turkey
Bahri Dagdas International Agricultural Research Institute
Konya Turkey
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur Turkey
Dokuz Eylül Üniversitesi
Izmir Turkey
Kastamonu University
Kastamonu Turkey
Mississippi State University
Mississippi State United States
Prairie View A&M University
Prairie View United States
Selçuk Üniversitesi
Selçuklu Turkey
TÜBİTAK Ulusal Metroloji Enstitüsü
Gebze Turkey
Metrikler
18
Atıf
12
Yazar
4
Anahtar Kelime