Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?
Reproduction in Domestic Animals · Temmuz 2023
YÖKSİS Kayıtları
Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?
Reproduction in Domestic Animals · 2023 SCI-Expanded
DOÇENT FATMA SATILMIŞ →
Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?
Reproduction in Domestic Animals · 2023 SCI-Expanded
PROFESÖR HÜSEYİN ERDEM →
Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?
Wiley · 2023 SCI-Expanded
DOÇENT KÜBRA KARAKAŞ ALKAN →
Does Using Microfluidic Sperm Sorting Chips in Bovine IVEP Affect Blastocyst Development?
Reproduction Domestic Animals · 2023 SCI-Expanded
DOÇENT HASAN ALKAN →
Does using microfluidic sperm sorting chips in bovine IVEP affect blastocyst development?
Wiley · 2023 SCI-Expanded
DOKTOR ÖĞRETİM ÜYESİ MUSTAFA BODU →
Makale Bilgileri
DergiReproduction in Domestic Animals
Yayın TarihiTemmuz 2023
Cilt / Sayfa58 · 1012-1020
Scopus ID2-s2.0-85161443259
Erişim🔓 Açık Erişim
Özet
This study aimed to determine the effect of microfluidic sperm sorting chip on embryo development and quality in the sperm treatment step in in vitro embryo production in cattle. Only A-quality oocytes obtained from the ovaries of Holstein cattle were included in the study. These oocytes were first placed in in vitro maturation medium, and matured oocytes were randomly divided into two groups at the 24th hour of maturation. Oocytes in the first group with the Microfluidic Sperm Sorting Chip (MFSC, n = 154) were put into a fertilization medium with spermatozoa prepared with microfluidic sperm sorting device. Oocytes in the second group (Con, n = 169) were fertilized with spermatozoa prepared by the routine sperm treatment step of the commercial company. The rate of cleavage (85.71% vs. 76.33%, respectively) and of reaching the blastocyst (44.15% vs. 32.54%, respectively) in the MFSC group were higher than the control group. In addition, it was determined that the numbers of ICM (45.8 ± 2.04 vs. 39.2 ± 1.85, respectively), TE (122.13 ± 2.19 vs. 115.0 ± 2.61, respectively), TC (167.93 ± 2.89 vs. 154.2 ± 2.62, respectively) increased in the MFSC group compared to the control group. A statistically significant difference was found in the number of cells with apoptosis per embryo (5.14 ± 0.77 vs. 11.91 ± 0.79) and apoptotic index rates (3.06 ± 0.47 vs. 7.72 ± 0.55%) of the MFSC and Con groups. As a result, we concluded that using microfluidic sperm sorting chips during sperm treatment in bovine IVEP increases the rate of reaching the blastocyst, embryo development, and quality and reduces the possibility of apoptosis in developing blastocysts. For this reason, it is also thought that the use of microfluidic sperm sorting devices during sperm treatment in bovine IVEP may be a new alternative in this field.
Yazarlar (9)
1
Hasan Alkan
ORCID: 0000-0001-8332-5334
2
Fatma Satilmiş
3
Mehmet Akif Demirel
4
Mustafa Bodu
ORCID: 0000-0002-8432-7347
5
Omer Faruk Yesilkaya
ORCID: 0000-0002-7721-2576
6
Muhammed Furkan Ciftci
ORCID: 0000-0001-8333-6500
7
Hüseyin Erdem
8
Mustafa Agah Tekindal
ORCID: 0000-0002-4060-7048
9
Kubra Karakas Alkan
Anahtar Kelimeler
apoptosis
cleavage rate
IVEP
rate of reaching blastocyst
sperm treatment
Kurumlar
İzmir Kâtip Çelebi Üniversitesi
Izmir Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
1
Atıf
9
Yazar
5
Anahtar Kelime