Scopus
YÖKSİS Eşleşti
Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester
Bioresource Technology · Ocak 2011
YÖKSİS Kayıtları
Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester
Bioresource Technology · 2011 SSCI 8 atıf
PROFESÖR MUSTAFA YILMAZ →
Immobilization of Candida rugosa lipase on glass beads for enantioselective hydrolysis of racemic Naproxen methyl ester
Bioresource Technology · 2011 SCI-Expanded
PROFESÖR ELİF ÖZYILMAZ →
Makale Bilgileri
DergiBioresource Technology
Yayın TarihiOcak 2011
Cilt / Sayfa102 · 499-506
Scopus ID2-s2.0-78650707084
Özet
Candida rugosa lipase (CRL) was immobilized on glutaraldehyde-activated aminopropyl glass beads by using covalent binding method or sol-gel encapsulation procedure and improved considerably by fluoride-catalyzed hydrolysis of mixtures of RSi(OCH3)3 and Si(OCH3)4. The catalytic properties of the immobilized lipases were evaluated into model reactions, i.e. the hydrolysis of p-nitrophenylpalmitate (p-NPP). It has been observed that the percent activity yield of the encapsulated lipase was 166.9, which is 5.5 times higher than that of the covalently immobilized lipase. The enantioselective hydrolysis of racemic Naproxen methyl ester by immobilized lipase was studied in aqueous buffer solution/isooctane reaction system and it was noticed that particularly, the glass beads based encapsulated lipases had higher conversion and enantioselectivity compared to covalently immobilized lipase. In short, the study confirms an excellent enantioselectivity (E>400) for the encapsulated lipase with an ee value of 98% for S-Naproxen. © 2010 Elsevier Ltd.
Yazarlar (4)
1
Elif Ozyilmaz
2
Keziban Can
3
Mehmet Sezgin
4
Mustafa Yilmaz
Anahtar Kelimeler
Candida rugosa lipase
Enantioselective hydrolysis
Glass beads
Immobilization
S-Naproxen
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
91
Atıf
4
Yazar
5
Anahtar Kelime