Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Utilization of cryopreserved ruminal liquor in in vitro gas production technique for evaluating nutritive value of some feedstuffs
Kafkas Universitesi Veteriner Fakultesi Dergisi · Mart 2017
YÖKSİS Kayıtları
Utilization of Cryopreserved Ruminal Liquor in In Vitro Gas ProductionTechnique for Evaluating Nutritive Value of Some Feedstuffs
Kafkas Universitesi Veteriner Fakultesi Dergisi · 2017 SCI-Expanded
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Makale Bilgileri
DergiKafkas Universitesi Veteriner Fakultesi Dergisi
Yayın TarihiMart 2017
Cilt / Sayfa23 · 325-332
Scopus ID2-s2.0-85032113627
Erişim🔓 Açık Erişim
Özet
In vitro gas production technique (IVGPT) is a routine method in nutritional sciences to determine energy content, organic matter (OM) digestibility, and fermentation kinetics of feedstuffs. After collecting from two ruminally cannulated Holstein heifers (350 kg), rumen liquors were used either fresh or cryopreserved form in the inoculums for IVGPT. Starch- (barley, wheat, and corn) and protein-rich (sunflower meal, cotton seed meal, and soybean meal) feedstuffs were evaluated for gas production kinetics, fermentation pattern, and energy content in 5 replicates. pH, NH3-N concentration and volatile fatty acids (VFA) profile, gas production, and fermentation kinetics parameters were measured. Data were analyzed by 2-way ANOVA. Viable protozoa rate was found to be 70.8% in cryopreserved rumen liquor after thawing. Decrease in pH in thawed rumen liquor was less than fresh rumen liquor as the incubation period advanced. Utilization of frozen rumen liquor after thawing in IVGPT was associated with lower VFA and NH3-N concentration, cumulative gas production, and metabolisable energy estimate for all feedstuffs. In conclusion, despite high correlation between in vitro data obtained from fresh and thawed rumen liquors to predict gas production, further experiments should cope with improving cryopreservation protocol for rumen liquors in order to optimize microbial activity for maintaining fermentation pattern.
Yazarlar (5)
1
Nurettin Gülşen
ORCID: 0000-0002-8555-0743
2
Huzur Derya Arık
3
Armaǧan Hayirli
4
Mustafa Selçuk Alataş
ORCID: 0000-0001-9920-1838
5
Melih Aksoy
Anahtar Kelimeler
Cryopreserved rumen liquor
Energy prediction
Fermentation
Gas production
In vitro gas test
Volatile fatty acid
Kurumlar
Atatürk Üniversitesi
Erzurum Turkey
Aydin Adnan Menderes University
Aydin Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
5
Yazar
6
Anahtar Kelime