Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis
Kafkas Universitesi Veteriner Fakultesi Dergisi · Ocak 2022
YÖKSİS Kayıtları
Comparison of Histopathological, Immunohistochemical and Real-Time PCR
Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis.
Kafkas Universitesi Veteriner Fakultesi Dergisi · 2022 SCI-Expanded
DOÇENT MEHMET BURAK ATEŞ →
Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI · 2022 TR-DİZİN, CABI, EBSCO, Scobus
PROFESÖR MUSTAFA ORTATATLI →
Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI · 2022 scopus, cabi, Google scholar, sci
PROFESÖR ÖZGÜR ÖZDEMİR →
Comparison of Histopathological, Immunohistochemical and Real-Time PCR Methods for Diagnosis of Listeriosis in Ruminants with Encephalitis
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI · 2022 Scopus, Ebsco
PROFESÖR FATİH HATİPOĞLU →
Makale Bilgileri
DergiKafkas Universitesi Veteriner Fakultesi Dergisi
Yayın TarihiOcak 2022
Cilt / Sayfa28 · 643-652
Scopus ID2-s2.0-85139398236
Erişim🔓 Açık Erişim
Özet
Encephalitic listeriosis is the most significant purulent encephalitis in ruminants and is a very common endemic problem in sheep, cattle, and goats. In this study, it was aimed to compare the presence of Listeria (L.) monocytogenes revealed by immunohistochemical (IHC) and Real-Time PCR methods with histopathological findings obtained from the archive materials. The study material consisted of pons and medulla oblongata paraffin tissue of 100 ruminants (9 cattle, 4 calves, 44 sheep, 38 lambs, and 5 goats). Positivity was obtained by the IHC method in 46 (46%) and by the Real-Time PCR method in 21 (21%) of 100 cases. In the L. monocytogenesis antigen IHC scoring, more severe staining was observed in sheep and goats (P>0.05). In the IHC positive cases, microabscess was more severe in sheep and goats than in cattle and lambs (P<0.05). In addition, 19 patients had Coenurus cerebralis cysts, and 3 of them were found to be positive for the IHC agent of Listeria. It was concluded that IHC and PCR methods can be used to detect L. monocytogenes from paraffin blocks, but the IHC method is a more effective method than PCR in revealing the presence of antigen from paraffin blocks stored for many years.
Yazarlar (6)
1
Fatih Hatipoglu
2
Funda Terzi
ORCID: 0000-0002-6184-5408
3
O. Ozdemir
4
M. Ortatatli
5
Mustafa Kemal Çiftçi
6
Mehmet Burak Ates
ORCID: 0000-0003-1297-426X
Anahtar Kelimeler
Histopathology
Immunohistochemistry
Listeriosis
Real-Time PCR
Ruminants
Kurumlar
İstanbul Gelişim Üniversitesi
Istanbul Turkey
Kastamonu University
Kastamonu Turkey
Kyrgyz-Turkish Manas University
Bishkek Kyrgyzstan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
2
Atıf
6
Yazar
5
Anahtar Kelime