Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Effect of Tarantula cubensis alcoholic extract and Nerium oleander distillate on cell proliferation markers in colon carcinogenesis
Revista Cientifica de la Facultad de Veterinaria · Ocak 2022
YÖKSİS Kayıtları
Effect of Tarantula cubensis alcoholic extract and Nerium oleander distillate on cell proliferation markers in colon carcinogenesis
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS · 2022 SCI-Expanded
PROFESÖR MEHMET TUZCU →
Effect of Tarantula cubensis alcoholic extract and Nerium oleander distillate on cell proliferation markers in colon carcinogenesis
Revista Científica de la Facultad de Ciencias Veterinarias · 2022 SCI-Expanded
PROFESÖR ÖZGÜR ÖZDEMİR →
Makale Bilgileri
DergiRevista Cientifica de la Facultad de Veterinaria
Yayın TarihiOcak 2022
Cilt / Sayfa32
Scopus ID2-s2.0-85172862145
Erişim🔓 Açık Erişim
Özet
Colorectal Cancer (CRC) is defined as colon and rectum cancer and is among the major causes of mortality in developed Countries. Tarantula cubensis alcoholic extract (TCAE) and Nerium oleander distillate (NOD) are reported to have anticancer and antioxidative activity. In this study, it was aimed to research the impact on cell proliferation markers of TCAE and NOD given simultaneously in experimental colon cancer. A total of 24 rats, 6 in each group, were used in the study. Cancer Control (CC): Azoxymethane was administered at the beginning of the experiment at a dose of 15 miligrams (mg)· kilograms-1 (kg), (Subcutaneous, SC) twice, with an interval of a week (wk), to induce cancer. CC+TCAE: the dosage of Azoxymethane administered was 15 mg·kg-1 (SC) twice a wk at the beginning of the experiment, while in the case of TCAE, it was 0.2 mL·kg-1 (SC) once a wk for 18 wk from the beginning of the experiment. Fifteen mg·kg-1 (SC) of Azoxymethane was administered twice at one-wk intervals at the beginning of the experiment to the CC+NOD group, and NO distillate (NOD) was given with water throughout the experiment. Afterwards, animals were euthanized under appropriate conditions, paraffin blocks formed from colon tissues, histochemical AgNOR (Silverstained nucleolar organizer regions), and immunohistochemical PCNA (proliferating cell nuclear antigen) stainings were performed. In the study, immunohistochemically, PCNA scores and AgNOR count per nucleus (AgNCI) were significantly decreased in C-TCAE and C-NOD groups (P<0.001). AgNOR Area index (AgNAI) (P<0.01), Core Area Index (CAI) (P<0.05), and AgNOR Area index/Core Area Index (AgNAI/CAI) (P<0.01) scores were significantly decreased in the C-TCAE group. As a result, it was concluded that both TCAE and NOD are effective as chemopreventive drugs and that TCAE presents a more pronounced antiproliferative effect than NOD.
Yazarlar (3)
1
O. Ozdemir
2
G. Akcakavak
3
Mehmet Tuzcu
Anahtar Kelimeler
AgNOR
Azoxymethane
immunohistochemistry
pathology
PCNA
Kurumlar
Bozok Üniversitesi
Yozgat Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
9
Atıf
3
Yazar
5
Anahtar Kelime