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Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)acetyltransferase in brain ischemia-reperfusion in rats

Iranian Journal of Basic Medical Sciences · Mayıs 2022

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YÖKSİS Kayıtları
Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats
Iranian Journal of Basic Medical Sciences · 2022 SCI-Expanded
PROFESÖR ESMA MENEVŞE →
Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats
Iran J Basic Med Sci · 2022 SCI
PROFESÖR RASİM MOĞULKOÇ →
Role of exogenous putrescine in the status of energy, DNA damage, inflammation, and spermidine/spermine-n(1)- acetyltransferase in brain ischemia-reperfusion in rats
Iranian journal of basic medical sciences · 2022 SCI
PROFESÖR ABDULKERİM KASIM BALTACI →

Makale Bilgileri

DergiIranian Journal of Basic Medical Sciences
Yayın TarihiMayıs 2022
Cilt / Sayfa25 · 597-603
Özet Objective(s): This study aims to investigate the role of putrescine against brain ischemia-reperfusion (IR) injured rats administered with 250 µmol/kg exogenous putrescine and highlight the IR-associated mechanisms in energy metabolism and inflammatory pathway. Materials and Methods: The rats were divided into six groups: 1-Sham group; 2-IR group, 30 min of ischemia and 30 min of reperfusion was performed with bilateral carotid occlusion (BCAO); 3-IPR group, a single oral dose of putrescine was administered at the start of the 30-minute reperfusion; while in the other treatment groups, 4 doses of putrescine were given within 12-hour intervals. After 30 min of reperfusion, the first dose was administered immediately in the IR-PI (group 4), after 3 hr in IR-PII (group 5), and after 6 hr in IR-PIII (group 6). Interleukin-6 (IL-6), Nuclear factor NF-kappa-B (NF-kB), Adenosine triphosphate (ATP), total Nitric oxide (NO), 8-hydroxyguanosine (8-OHdG), Spermidine/Spermin N-acetyltransferase (SSAT) levels were analyzed in brain tissues. Results: IR reduced brain ATP levels; however, putrescine treatment reversed this state. Brain NO and 8-OHdG levels, and NF-kB and IL-6 levels increased significantly in the IR group and these elevations were decreased in putrescine administered groups. SSAT levels were higher in the IR-PII group. The lowest levels were observed in the IR-PIII group. Conclusion: The exogenous putrescine supplementation after cerebral IR creates neuroprotective effects independent of the time of administration; according to conditions such as formation of radicals in the brain, the spread of the inflammation and the need for consumption of energy are considered as a whole.

Yazarlar (5)

1
Nihal Cetin
2
Dervis Dasdelen
3
Rasim Mogulkoc
4
Esma Menevşe
5
Abdulkerim Kasim Baltaci

Anahtar Kelimeler

8-OHdG Brain ischemia IL-6 NF-kB Nitric oxide Putrescine Rat Spermidine/Spermine-N(1)

Kurumlar

Selçuk Tip Fakültesi
Konya Turkey

Metrikler

7
Atıf
5
Yazar
8
Anahtar Kelime