Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
The usability of cytological and immunocytological methods for rapid diagnosis of encephalitic listeriosis in ruminants
Kafkas Universitesi Veteriner Fakultesi Dergisi · Ocak 2021
YÖKSİS Kayıtları
Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması
Kafkas Universitesi Veteriner Fakultesi Dergisi · 2021 SCI-Expanded
DOÇENT MEHMET BURAK ATEŞ →
The Usability of Cytological and Immunocytological Methods for Rapid Diagnosis of Encephalitic Listeriosis in Ruminants (Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması)
Kafkas Universitesi Veteriner Fakultesi Dergisi · 2021 CABI, EBSCO, Scobus
PROFESÖR MUSTAFA ORTATATLI →
Ruminantlarda Ensefalitik Listeriozisin Hızlı Tanısı İçin Sitolojik ve İmmunositolojik Yöntemlerin Kullanılabilirliğinin Araştırılması
Kafkas Universitesi Veteriner Fakultesi Dergisi · 2021 SCI-Expanded
PROFESÖR ÖZGÜR ÖZDEMİR →
The Usability of Cytological and Immunocytological Methods for Rapid Diagnosis of Encephalitic Listeriosis in Ruminants
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI · 2021 SCI-Expanded
PROFESÖR FATİH HATİPOĞLU →
Makale Bilgileri
DergiKafkas Universitesi Veteriner Fakultesi Dergisi
Yayın TarihiOcak 2021
Cilt / Sayfa27 · 225-233
Scopus ID2-s2.0-85102377352
Erişim🔓 Açık Erişim
Özet
Although the clinical and pathological findings are important in the diagnosis of listeriosis, to isolation or to be shown the presence of the bacterium must be required for the definitive diagnosis. This study aims to investigate the availability of imprint cytological (IC) and immunocytochemical (ICC) methods in comparison with histopathological and immunohistochemical (IHC) methods for the rapid diagnosis of encephalitic listeriosis. In the study, the touching and smear preparations taken from the pons and medulla oblongata of 25 ruminants suspected with listeriosis by neurological symptoms were stained with modified giemsa and also with ICC technic for revealing antigens, as a new method. Same tissue sections were stained with Hematoxylin&Eosin and IHC methods too, and examined under light microscope by scoring. In IC examinations, there were intensive neutrophils in 14 cases and few neutrophils in 4 cases, and no neutrophils were observed in 7 cases. In histopathological examinations, 13 of these 14 cases revealed typical microabscesses and listeria positivity in IHC staining. ICC positivity was detected in 12 (92.3%) of the listeria positive 13 cases. A highly positive correlation was observed among cytology (14), ICC (12), histopathology and IHC (13) scores (r2> 0.8; P<0.01). In conclusion, the cytological examination of the pons and medulla oblongata of listeriosis-suspected ruminants revealed that a rapid pre-diagnosis could be made with the presence of intense neutrophils. Also, with ICC staining of cytological preparations, the diagnosis could be performed with 92.3% accuracy. Since ICC is an easy and fast method, it is concluded that it can be used safely especially in field studies, along with cytological examination.
Yazarlar (6)
1
O. Ozdemir
2
M. Ortatatli
3
Funda Terzi
ORCID: 0000-0002-6184-5408
4
Fatih Hatipoglu
5
Mustafa Kemal Çiftçi
6
Mehmet Burak Ates
ORCID: 0000-0003-1297-426X
Anahtar Kelimeler
Cytology
Histopathology
Immunocytology
Immunohistochemistry
Listeriosis
Kurumlar
Kastamonu University
Kastamonu Turkey
Kyrgyz-Turkish Manas University
Bishkek Kyrgyzstan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
4
Atıf
6
Yazar
5
Anahtar Kelime