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Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

Phytochemistry · Haziran 2022

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YÖKSİS Kayıtları
Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress
Phytochemistry · 2022 SCI-Expanded
DOÇENT BÜŞRA ARIKAN ABDULVELİ →
Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress
Phytochemistry · 2022 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ FATMA NUR ALP TURGUT →
Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress
Phytochemistry · 2022 SCI-Expanded
PROFESÖR EVREN YILDIZTUGAY →

Makale Bilgileri

DergiPhytochemistry
Yayın TarihiHaziran 2022
Cilt / Sayfa198
Özet The impacts of exogenous rosmarinic acid (RA, 100 μM) and/or hesperidin (HP, 100 μM) were evaluated in improving tolerance on the gas exchange, chlorophyll fluorescence and efficiencies, phenomenological fluxes of photosystems, antioxidant system and gene expression related to the lipid biosynthesis under heat stress. For this purpose, Arabidopsis thaliana was grown under RA and HP with heat stress (S, 38 °C) for 24 h(h). As shown in gas exchange parameters, heat stress caused mesophyll efficiency and non-stomatal restrictions. Both alone and combined forms of RA and HP to stress-treated A. thaliana alleviated the disturbance of carbon assimilation, transpiration rate and internal CO2 concentrations. Stress impaired the levels of energy flow reaching reaction centers of PSII and the photon capture ability of active reaction centers. RA and/or HP enhanced photosystems' structural/functional characteristics and photosynthetic performance. Histochemical staining and biochemical analyses revealed that heat stress caused the oxidation in A. thaliana. By activating several defensive mechanisms, RA and/or HP could reverse the harm caused by radical production. Both alone and combined forms of RA and HP removed superoxide anion radical (O2•−) accumulation, inducing superoxide dismutase (SOD). The common enzyme that scavenged hydrogen peroxide (H2O2) at all three applications (S + RA, S + HP and S + RA + HP) was POX. Also, only RA could utilize the ascorbate (AsA) regeneration in response to stress, suggesting increased ascorbate peroxidase (APX), monodehydroascorbate (MDHAR) and dehydroascorbate (DHAR) activities. However, the regeneration/redox state of AsA and glutathione (GSH) did not maintain under S + HP and S + RA + HP. While RA had no positive influence on the saturated fatty acids under stress, HP increased the total saturated fatty acids (primarily palmitic acid). Besides, the combined application of RA + HP effectively created the stress response by increasing the expression of genes involved in fatty acid synthesis. The synergetic interactions of RA and HP could explain the increased levels of saturated fatty acids in combining these compounds. The data obtained from the study will contribute to the responses of phenolic compounds in plants to heat stress.

Yazarlar (5)

1
Busra Arikan
ORCID: 0000-0001-5313-0501
2
Ceyda Ozfidan-Konakci
ORCID: 0000-0002-7134-0948
3
Fatma Nur Alp-Turgut
ORCID: 0000-0002-1884-2367
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Evren Yildiztugay
ORCID: 0000-0002-4675-2027

Anahtar Kelimeler

Arabidopsis thaliana Brassicaceae Gene expression Heat stress Hesperidin Rosmarinic acid

Kurumlar

Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey

Metrikler

10
Atıf
5
Yazar
6
Anahtar Kelime