Scopus
YÖKSİS Eşleşti
BoLA-DRB3.2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · Ocak 2023
YÖKSİS Kayıtları
BoLA-DRB3.2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · 2023 CABI
PROFESÖR OĞUZHAN AVCI →
BoLA-DRB3.2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · 2023 SCI-Expanded
DOÇENT IRMAK DİK →
BoLA-DRB3. 2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · 2023 SCI-Expanded
PROFESÖR ATİLLA ŞİMŞEK →
BoLA-DRB3.2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · 2023 SCI-Expanded
PROFESÖR ORHAN YAPICI →
BoLA-DRB3.2 Gene Polymorphism in Cattle Naturally Infected with Bovine Leukemia Virus
Pakistan Veterinary Journal · 2023 SCI-Expanded
PROFESÖR SİBEL YAVRU →
Makale Bilgileri
DergiPakistan Veterinary Journal
Yayın TarihiOcak 2023
Cilt / Sayfa43 · 174-178
Scopus ID2-s2.0-85169506318
Özet
This study was conducted to determine and evaluate the BoLA-DRB3.2 polymorphisms in bovine leukemia virus (BLV) seropositive cows by DNA Sequence Analysis. Isolates of DNA obtained from seropositive (25) and seronegative blood samples (3) collected from cows between 2005 and 2010 were used in this study. DNA isolation was performed from blood samples. MHC analyses were performed using primers belonging to BoLA-DRB3.2 genes. DNA sequence analyses were performed. The number and diversity of haplotypes and nucleotide diversity were calculated. The phylogenetic analysis was determined that cows had 11 haplotypes, healthy individuals were associated together in the first haplotype, while in other haplotypes seropositive cows were associated among themselves and there was 1 seropositive cow between haplotype 6 and 11. Also, the variety of haplotypes (Hd) (0.847), total nucleotide variety (π) (0.033), variation rate (0.00285), and Tajima's D statistical value (0.103) were detected. When FST of individuals were evaluated, it was revealed that healthy individuals were genetically distant from positive individuals. In this study, it was observed that BLV seropositive individuals differed genetically compared to healthy animals. In conclusion, BoLA-DRB3.2 gene polymorphism can be considered as a supporting parameter in BLV diagnosis.
Yazarlar (11)
1
Oğuzhan Avci
ORCID: 0000-0001-9299-4695
2
Oya Bulut
3
Irmak Dik
ORCID: 0000-0003-2516-9489
4
Zafer Bulut
5
Müge Dogan
6
M. Kale
7
Sibel Hasircioglu
8
Orhan Yapıcı
9
Atilla Şimşek
10
Sibel Yavru
11
Yakup Sinan Orta
Anahtar Kelimeler
BoLA-DRB3.2
Bovine leukemia virus
Cattle
Gene polymorphism
Kurumlar
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur Turkey
Dokuz Eylül Üniversitesi
Izmir Turkey
Selçuk Üniversitesi
Selçuklu Turkey
T.C. Tarim ve Köyişleri Bakanliği
Antakya Turkey