Scopus
YÖKSİS Eşleşti
Superior photo-induced antibacterial/antibiofilm activities of ZnPcs/TiO<inf>2</inf> and computational simulation studies
Journal of Materials Chemistry B · Haziran 2023
YÖKSİS Kayıtları
Superior photo-induced antibacterial/antibiofilm activities of ZnPcs/TiO2 and computational simulation studies
Journal of Materials Chemistry B · 2023 SCI-Expanded
DOÇENT EMRE ASLAN →
Superior photo-induced antibacterial/antibiofilm activities of ZnPcs/TiO2 and computational simulation studies
Journal of Materials Chemistry B · 2023 SCI-Expanded
PROFESÖR İMREN HATAY PATIR →
Superior photo-induced antibacterial/antibiofilm activities of ZnPcs/TiO2 and computational simulation studies
Journal of Materials Chemistry B · 2023 SCI-Expanded
ARAŞTIRMA GÖREVLİSİ İLKNUR AKSOY ÇEKCEOĞLU →
Makale Bilgileri
DergiJournal of Materials Chemistry B
Yayın TarihiHaziran 2023
Cilt / Sayfa11 · 6361-6371
Scopus ID2-s2.0-85163715121
Özet
Bacteria can form biofilms on any surface, which causes biofilm-associated infections and bacterial resistance to antibiotics. Thus, it is important to design new-generation non-chemotherapeutic nanoagents for effective antibacterial and antibiofilm strategies. Herein, the effects of the anchoring groups, which are imidazole and carboxylic acid, of zinc phthalocyanines (ZnPcs) sensitized TiO2 on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) were investigated under light-emitting diode (LED) irradiation. The photocatalytic antibacterial activities of ZnPc-1/TiO2 and ZnPc-2/TiO2 on the bacterial strains were examined by monitoring the optical density value at 600 nm (OD600 nm). Glutathione (GSH) oxidation assay was used to measure the reactive oxygen species (ROS) generation activity of the compounds. Bacterial damages were imaged by scanning electron microscopy (SEM). According to our photocatalytic antibacterial mechanism, photogenerated electrons are transferred from Pcs to TiO2 and then react with O2, thus creating ROS, which causes damage to bacterial membrane, protein and biofilm destruction as well. Further, computational simulation analysis was used to show the interaction patterns of ZnPc-1 and ZnPc-2 with penicillin binding protein 2a (PBP2a) of S. aureus and FimH lectin protein (PDB:4XO8) of E. coli to elucidate the dark molecular antibacterial mechanism of the compounds. The obtained results from computational studies showed that ZnPc-2 binds firmly through bonds with the 1MWT protein from S. aureus. On the other hand, ZnPc-1 binds firmly through bonds with the 4XO8 protein from E. coli. From combining experimental and computational results, we can conclude that this strategy can be applied to different types of bacterial infections.
Yazarlar (9)
1
Tuğçe Özcan
ORCID: 0000-0001-5209-1950
2
İlknur Aksoy Çekceoğlu
ORCID: 0000-0001-9950-3967
3
Khattab Al-Khafaji
ORCID: 0000-0002-2165-9256
4
Erkan Öner
ORCID: 0000-0002-6332-6484
5
A. Celil Yüzer
6
Serap Yalın
7
Emre Aslan
8
Mine Ince
ORCID: 0000-0002-9164-0446
9
Imren Hatay Patir
ORCID: 0000-0003-2937-6557
Kurumlar
Adiyaman Üniversitesi
Adiyaman Turkey
Al-Karkh University of Science
Baghdad Iraq
Mersin Üniversitesi
Mersin Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Tarsus University
Tarsus Turkey
Metrikler
1
Atıf
9
Yazar