Scopus
YÖKSİS DOI Eşleşti
SJR Q2
Assessment of in vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil
Pharmacognosy Magazine · Ocak 2016
YÖKSİS Kayıtları
Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil
Pharmacognosy Magazine · 2016 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Effects of aqueous extract from Silybum marianum on adenosine deaminase activity in cancerous and noncancerous human gastric and colon tissues
2015 ISSN: 0973-1296 SCI-Expanded 1 atıf
Prof. Dr. BAHADIR ÖZTÜRK →
Assessment of In vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa Oil
2016 ISSN: 0973-1296 SCI-Expanded
Prof. Dr. BAHADIR ÖZTÜRK →
Assessment of In vitro antibacterial activity and cytotoxicity effect of Nigella sativa oil
2016 ISSN: 0973-1296 SCI-Expanded
Prof. Dr. HATİCE TÜRK DAĞI →
Assessment of in vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa Oil.
2016 ISSN: 0973-1296 SCI-Expanded Q4
Doç. Dr. GÜLSÜM ABUŞOĞLU →
Bioactivity-guided isolation of cytotoxic and antioxidant phytochemicals from four Cousinia species from stenocephala bunge section
2019 ISSN: 0973-1296 SCI-Expanded Q4
Prof. Dr. OSMAN TUGAY →
Makale Bilgileri
Dergi
Pharmacognosy Magazine
ISSN09731296
Yayın TarihiOcak 2016
Cilt / Sayfa12 · S471-S474
Scopus ID2-s2.0-85019634310
Özet
Background: Methicillin resistance is a serious health concern since it has spread among Staphylococcus aureus and coagulase-negative Staphylococci (CoNS) that are frequent community and nosocomial pathogens worldwide. Methicillin-resistant strains are often resistant to other classes of antibiotics, making their treatment difficult. Nigella sativa oil is known to be active against Gram-positive cocci, yet its in vitro cytotoxicity is rarely investigated, is a proper and powerful candidate for treatment of methicillin-resistant isolates. Objectives: The aim of this study is to evaluate the in vitro antibacterial activity and cytotoxicity effect of N. sativa oil. Materials and Methods: The minimal inhibitory concentrations (MICs) of N. sativa oil were determined by broth microdilution method against four different American Type Culture Collection strains, 45 clinical isolates of methicillin-resistant S. aureus (MRSA), and 77 methicillin-resistant CoNS (MRCoNS). The effects of different dilutions (0.25 μg/mL, 0.5 μg/mL, and 1 μg/mL) of N. sativa oil on the proliferation of gingival fibroblasts were evaluated. Results: The MIC values of N. sativa oil against clinical isolates of Staphylococci were between <0.25 μg/mL and 1.0 μg/mL. Compared to the control group, there was no cytotoxic effect on the proliferation of the gingival fibroblasts. Conclusion: In the present study, the oil of N. sativa was very active against MRSA and MRCoNS and had no in vitro cytotoxicity at relevant concentrations. These findings emphasize that there is a requirement for further clinical trials on N. sativa oil for "safe" medical management of infections caused by methicillin-resistant Staphylococci. Abbreviation used: ATCC: American Type Culture Collection; CLSI: Clinical and Laboratory Standards Institute; CoNS: Coagulase-negative Staphylococci; DMEM: Dulbecco′s modified Eagle′s medium; DMSO: Dimethyl sulfoxide; FBS: Fetal bovine serum; HGF: Human gingival fi broblast; MIC: Minimal inhibitory concentration; MRCoNS: Methicillin-resistant CoNS;MRSA: Methicillin-resistant S. aureus.
Yazarlar (5)
1
Ayse Ruveyda Ugur
2
Hatice Türk Dağı
3
Bahadır Öztürk
4
G. Tekin
5
Duygu Findik
Anahtar Kelimeler
Cytotoxic effect
methicillin-resistant Staphylococci
microdilution
Nigella sativa
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Pharmacognosy Magazine (discontinued)
Q2
OA
SJR Skoru0,345
H-Index58
YayıncıWolters Kluwer Medknow Publications
ÜlkeIndia
Pharmaceutical Science (Q2)
Drug Discovery (Q3)
Metrikler
49
Atıf
5
Yazar
4
Anahtar Kelime