Scopus
YÖKSİS DOI Eşleşti
SJR Q1
Determination of immunoreactive proteins of Babesia ovis
Veterinary Parasitology · Aralık 2013
YÖKSİS Kayıtları
Determination of immunoreactive proteins of Babesia ovis
Veterinary Parasitology · 2013 SCI-Expanded
Prof. Dr. NERMİN IŞIK USLU →
YÖKSİS Kayıtları — ISSN Eşleşmesi
Expression profile and polymorphisms of actin genes in protoscoleces of Echinococcus granulosus from sheep in central Turkey Doktora tez çalışmasından üretilmiştir
2009 ISSN: 0304-4017 SCI 1 atıf
Prof. Dr. HİLAL ARIKOĞLU →
A new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis Its molecular characterization subcellular localization and antibody recognition by infected sheep
2015 ISSN: 03044017 SCI-Expanded
Prof. Dr. FERDA SEVİNÇ →
Instability of ovine babesiosis in an endemic area in Turkey
2012 ISSN: 03044017 SCI-Expanded
Prof. Dr. FERDA SEVİNÇ →
Babesia ovis infections Detailed clinical and laboratory observations in the pre and post treatment periods of 97 field cases
2013 ISSN: 03044017 SCI-Expanded 5 atıf
Prof. Dr. NERMİN IŞIK USLU →
Determination of immunoreactive proteins of Babesia ovis
2013 ISSN: 03044017 SCI-Expanded
Prof. Dr. NERMİN IŞIK USLU →
Therapeutic and prophylactic efficacy of imidocarb dipropionate on experimental Babesia ovis infection of lambs
2007 ISSN: 03044017 SSCI 19 atıf
Prof. Dr. FERDA SEVİNÇ →
A comparative study on the prevalence of Theileria equi and Babesia caballi infections in horse sub populations in Turkey
2008 ISSN: 03044017 SSCI
Prof. Dr. MEHMET MADEN →
A new immunoreactive recombinant protein designated as rBoSA2 from Babesia ovis Its molecular characterization subcellular localization and antibody recognition by infected sheep
2015 ISSN: 0304-4017 SCI-Expanded
Doç. Dr. ONUR CEYLAN →
Haemoparasitic agents associated with ovine babesiosis: A possible negative interaction between Babesia ovis and Theileria ovis
2018 ISSN: 0304-4017 SCI-Expanded
Prof. Dr. MUTLU SEVİNÇ →
Endemic instability of ovine babesiosis in Turkey: A country-wide sero-epidemiological study
2020 ISSN: 0304-4017 SCI-Expanded
Prof. Dr. FERDA SEVİNÇ →
Untargeted metabolomics to discriminate liver and lung hydatid cysts: Importance of metabolites involved in the immune response
2024 ISSN: 0304-4017 SCI Q2
Prof. Dr. SALİH MAÇİN →
Classification of chicken Eimeria species through deep transfer learning models: A comparative study on model efficacy
2025 ISSN: 0304-4017 SCI-Expanded Q2
Doç. Dr. ONUR CEYLAN →
Makale Bilgileri
Dergi
Veterinary Parasitology
ISSN03044017
Yayın TarihiAralık 2013
Cilt / Sayfa198 · 391-395
Scopus ID2-s2.0-84888436145
Özet
Babesia ovis, an intraerythrocytic protozoan parasite transmitted by ticks, causes severe infections in sheep in tropical and subtropical regions of the world. Parasite-specific immunoreactive proteins have been used as antigen in the serological diagnosis of babesiosis. There is no study about determination of B. ovis-specific proteins in sheep. This study was planned to determine the immunoreactive proteins of B. ovis. In this study, two splenectomized lambs, and twelve seropositive sheep and five seronegative lambs for anti B. ovis antibodies were used as materials. Infected blood samples at 5% of parasitemia from the two splenectomized lambs experimentally infected with a virulent B. ovis field strain were analyzed for B. ovis-specific proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting (WB). B. ovis-specific five major proteins were recognized by anti B. ovis serum but not by healthy sheep serum. They were of approximate molecular weights 154, 109, 77, 58, and 38. kDa. As the control samples, protein profiles of the blood extracts of two lambs before splenectomy operation were also blotted with the immune sera, but none of the five proteins was detected. These proteins were also immunoblotted with heterologous positive and negative sheep sera. All of twelve positive sera recognized the 109. kDa protein with 100 percent sensitivity. The 77. kDa protein reacted in 11 of 12 sera (91.6%). The sensitivities of the other 3 proteins ranged between 83.3% and 25%. The five protein bands immunoblotted with sera of the 5 negative lambs did not give any positive reaction. The results of this study revealed the presence of proteins recognized by the serum antibodies of experimentally and naturally infected sheep with B. ovis. Additional studies on the purification of these proteins and on subsequently their utilization in a serodiagnostic method are required to improve the serological diagnosis of ovine babesiosis. © 2013 Elsevier B.V.
Yazarlar (5)
1
Ferda Sevinc
2
Leyla Guler
3
Mutlu Sevinc
4
Özlem Derinbay Ekici
5
Nermin Isik
Anahtar Kelimeler
Babesia ovis
Immunoreactive proteins
SDS-PAGE
Western Blotting
Kurumlar
Burdur Mehmet Akif Ersoy Üniversitesi
Burdur Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Scimago Dergi (ISSN Eşleşmesi)
Veterinary Parasitology
Q1
SJR Skoru0,606
H-Index150
YayıncıElsevier B.V.
ÜlkeNetherlands
Veterinary (miscellaneous) (Q1)
Medicine (miscellaneous) (Q2)
Parasitology (Q2)
Metrikler
9
Atıf
5
Yazar
4
Anahtar Kelime