Scopus
YÖKSİS Eşleşti
Molecular detection and genetic identification of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in Turkey
Ticks and Tick-borne Diseases · Şubat 2016
YÖKSİS Kayıtları
Molecular detection and genetic identification of Babesia bigemina Theileria annulata Theileria orientalis and Anaplasma marginale in Turkey
Ticks and Tick-borne Diseases · 2016 SCI-Expanded
DOÇENT ONUR CEYLAN →
Molecular detection and genetic identification of Babesia bigemina Theileria annulata Theileria orientalis and Anaplasma marginale in Turkey
Ticks and Tick-borne Diseases · 2016 SCI-Expanded
PROFESÖR FERDA SEVİNÇ →
Molecular detection and genetic identification of Babesia bigemina Theileria annulata Theileria orientalis and Anaplasma marginale in Turkey
Ticks and Tick-borne Diseases · 2016 SCI-Expanded
PROFESÖR FERDA SEVİNÇ →
Molecular detection and genetic identification of Babesia bigemina Theileria annulata Theileria orientalis and Anaplasma marginale in Turkey
Ticks and Tick-borne Diseases · 2016 SSCI
PROFESÖR MUTLU SEVİNÇ →
Makale Bilgileri
DergiTicks and Tick-borne Diseases
Yayın TarihiŞubat 2016
Cilt / Sayfa7 · 126-134
Scopus ID2-s2.0-84947466262
Özet
Babesia spp., Theileria spp. and Anaplasma spp. are significant tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of Babesia bigemina, Theileria annulata, Theileria orientalis and Anaplasma marginale in cattle from 6 provinces of Turkey using species-specific PCR assays. The PCR were conducted using the primers based on the B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. annulata merozoite surface antigen-1 (Tams-1), T. orientalis major piroplasm surface protein (ToMPSP) and A. marginale major surface protein 4 (AmMSP4) genes, respectively. Fragments of B. bigemina internal transcribed spacer (BbiITS), T. annulata internal transcribed spacer (TaITS), ToMPSP and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the overall infections of A. marginale, T. annulata, B. bigemina and T. orientalis were 29.1%, 18.9%, 11.2% and 5.6%, respectively. The co-infection of two or three pathogens was detected in 29/196 (15.1%) of the cattle samples. The results of sequence analysis indicated that BbiRAP-1a, BbiITS, Tams-1, ToMPSP and AmMSP4 were conserved among the Turkish samples, with 99.76%, 99-99.8%, 99.34-99.78%, 96.9-99.61% and 99.42-99.71% sequence identity values, respectively. In contrast, the Turkish TaITS gene sequences were relatively diverse with 92.3-96.63% identity values. B. bigemina isolates from Turkey were found in the same clade as the isolates from other countries in phylogenetic analysis. On the other hand, phylogenetic analysis based on T. annulata ITS sequences revealed significant differences in the genotypes of T. annulata isolates from Turkey. Additionally, the T. orientalis isolates from Turkish samples were classified as MPSP type 3 genotype. This is the first report of type 3 MPSP in Turkey. Moreover, AmMSP4 isolates from Turkey were found in the same clade as the isolates from other countries. This study provides important data for understanding the epidemiology of tick-borne diseases and it is expected to improve approach for diagnosis and control of tick-borne diseases in Turkey.
Yazarlar (13)
1
Mo Zhou
2
Shinuo Cao
3
Ferda Sevinc
4
Mutlu Sevinc
5
Onur Ceylan
6
Paul Franck Adjou Moumouni
7
Charoonluk Jirapattharasate
8
Mingming Liu
9
Guanbo Wang
10
Aiko Iguchi
11
Patrick Vudriko
12
Hiroshi Suzuki
13
Xuenan Xuan
Anahtar Kelimeler
Cattle
Species-specific PCR
Tick-borne pathogen
Turkey
Kurumlar
Obihiro University of Agriculture and Veterinary Medicine
Obihiro Japan
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
47
Atıf
13
Yazar
4
Anahtar Kelime