Scopus
YÖKSİS Eşleşti
Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC-DAD-ESI-Q-TOF-MS/MS) and Neurobiological Potential of Haplophyllum sahinii and H. vulcanicum Extracts
Chemistry and Biodiversity · Eylül 2019
YÖKSİS Kayıtları
Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS) and Neurobiological Potential ofHaplophyllum sahiniiandH. vulcanicumExtracts
Wiley · 2019 SSCI
ARAŞTIRMA GÖREVLİSİ ESENGÜL ÖZKAYMAKOĞLU →
Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC‐DAD‐ESI‐Q‐TOF‐MS/MS) and Neurobiological Potential of iHaplophyllum sahinii/i and iH. vulcanicum/i Extracts/title
CHEMISTRY BIODIVERSITY · 2019 SCI-Expanded
PROFESÖR OSMAN TUGAY →
Makale Bilgileri
DergiChemistry and Biodiversity
Yayın TarihiEylül 2019
Cilt / Sayfa16
Scopus ID2-s2.0-85070507547
Özet
In the current study, the ethanol extracts of flower, stem, and root parts of two endemic Turkish species, e. g., Haplophyllum sahinii O. Tugay & D. Ulukuş and H. vulcanicum Boiss. & Heldr., were screened against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) associated with Alzheimer's disease as well as tyrosinase (TYR) linked to Parkinson's disease using ELISA microplate assay at 200 μg/mL. Among the extracts, the highest inhibition was caused by the stem extract of H. sahinii against BChE (IC50=64.93±1.38 μg/mL). Consistently, all of the extracts were found to exert a selective inhibition towards BChE to some extent. It was only the root extract of H. vulcanicum that could inhibit AChE at low level (IC50=203.18±5.33 μg/mL). None of the extracts displayed an inhibition over 50 % against TYR. Metabolite profiling of the extracts was achieved by a highly hyphenated liquid chromatographic mass spectrometric technique (HPLC-DAD-ESI-Q-TOF-MS/MS), which revealed the presence of furoquinoline (β-fagarine, γ-fagarine) and amide (tubasenicine, tubacetine) alkaloids; furano- (rutamarin), pyrano- (xanthyletine), and geranyloxy coumarins; phenylpropanoid (secoisolariciresinol), arylnaphthalene (mono-O-acetyldiphyllin apioside), and dibenzylbutyrolactone (kusunokinin, haplomyrfolin) lignans. Several important differences were observed between the extracts analyzed. β-Fagarine was the major alkaloid in H. vulcanicum, whereas γ-fagarine was present only in the roots of both Haplophyllum species; moreover, secoisolariciresinol and secoisolariciresinol dimethyl ether were the main lignans in the stems and flowers. This is the first study identifying ChE and TYR inhibitory effect and metabolic profiles of H. vulcanicum and H. sahinii.
Yazarlar (7)
1
Esengul Karahisar
2
Osman Tugay
3
Ilkay Erdogan Orhan
4
F. Sezer Senol
5
Simon Vlad Luca
6
Krystyna Skalicka-Wozniak
7
Mustafa Şahin
Anahtar Kelimeler
biological activity
cholinesterase
Haplophyllum sahinii
Haplophyllum vulcanicum
metabolite profiling
tyrosinase
Kurumlar
Gazi Üniversitesi
Ankara Turkey
Grigore T. Popa University of Medicine and Pharmacy of Iaºi
Iasi Romania
Medical University of Lublin
Lublin Poland
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
8
Atıf
7
Yazar
6
Anahtar Kelime