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Microwave-assisted extraction, HPLC analysis, and inhibitory effects on carbonic anhydrase I, II, VA, and VII isoforms of 14 blueberry Italian cultivars

Journal of Enzyme Inhibition and Medicinal Chemistry · Kasım 2016

Makale Bilgileri

DergiJournal of Enzyme Inhibition and Medicinal Chemistry
Yayın TarihiKasım 2016
Cilt / Sayfa31 · 1-6
Erişim🔓 Açık Erişim
Özet The multi-component fingerprint and the biological evaluation of plant-derived material are indispensable for the pharmaceutical field, in food quality control procedures, and in all plant-based products. We investigated the quantitative content of biologically active compounds (anthocyanins and chlorogenic acid) of microwave-assisted blueberry extracts from 14 different Italian cultivars, using validated high-performance liquid chromatography-photodiode array detector (HPLC-PDA) method and routinely instrument configuration. The carbonic anhydrase (CA, EC 4.2.1.1) inhibition profiles against several pharmacologically relevant CA isoforms of blueberry extracts and some bioactive compounds were also investigated. The various cultivars showed a highly variable content in anthocyanins and chlorogenic acid, and their CA inhibitory effects were also highly variable. Overall these data prove that antioxidant natural products found in blueberries may be useful for designing pharmacological agents in which various CAs are involved, e.g., antiobesity, antitumor, or anticonvulsants agents.

Yazarlar (11)

1
Adriano Mollica
2
Marcello Locatelli
ORCID: 0000-0002-0840-825X
3
Giorgia Macedonio
4
Simone Carradori
5
Anatoly Petrovich Sobolev
6
Roberto F. De Salvador
7
Simona M. Monti
8
Martina Buonanno
9
Gokhan Zengin
ORCID: 0000-0001-6548-7823
10
Andrea Angeli
11
Claudiu T. Supuran
ORCID: 0000-0003-4262-0323

Anahtar Kelimeler

Blueberry carbonic anhydrase inhibitors cultivar HPLC-PDA microwave-assisted extraction

Kurumlar

Consiglio Nazionale delle Ricerche
Rome Italy
Consiglio per la Ricerca in Agricoltura e l’Analisi Dell’Economia Agraria (CREA)
Rome Italy
Istituto di Biostrutture e Bioimmagini
Naples Italy
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi della Campania Luigi Vanvitelli
Naples Italy
Università degli Studi di Firenze
Florence Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy

Metrikler

53
Atıf
11
Yazar
5
Anahtar Kelime

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