Scopus
🔓 Açık Erişim
Analysis of imidazoles and triazoles in biological samples after MicroExtraction by packed sorbent
Journal of Enzyme Inhibition and Medicinal Chemistry · Ocak 2017
Makale Bilgileri
DergiJournal of Enzyme Inhibition and Medicinal Chemistry
Yayın TarihiOcak 2017
Cilt / Sayfa32 · 1053-1063
Scopus ID2-s2.0-85026870702
Erişim🔓 Açık Erişim
Özet
This paper reports the MEPS-HPLC-DAD method for the simultaneous determination of 12 azole drugs (bifonazole, butoconazole, clotrimazole, econazole, itraconazole, ketoconazole, miconazole, posaconazole, ravuconazole, terconazole, tioconazole and voriconazole) administered to treat different systemic and topical fungal infections, in biological samples. Azole drugs separation was performed in 36 min. The analytical method was validated in the ranges as follows: 0.02–5 μg mL−1 for ravuconazole; 0.2–5 μg mL−1 for terconazole; 0.05–5 μg mL−1 for the other compounds. Human plasma and urine were used as biological samples during the analysis, while benzyl-4-hydroxybenzoate was used as an internal standard. The precision (RSD%) and trueness (Bias%) values fulfill with International Guidelines requirements. To the best of our knowledge, this is the first HPLC-DAD procedure coupled to MEPS, which provides the simultaneous analysis of 12 azole drugs, available in the market, in human plasma and urine. Moreover, the method was successfully applied for the quantitative determination of two model drugs (itraconazole and miconazole) after oral administration in real samples.
Yazarlar (10)
1
Cristina Campestre
2
Marcello Locatelli
ORCID: 0000-0002-0840-825X
3
Paolo Guglielmi
4
Elisa De Luca
5
Giuseppe Bellagamba
6
Sergio Menta
7
Gokhan Zengin
ORCID: 0000-0001-6548-7823
8
Christian Celia
9
Luisa Di Marzio
10
Simone Carradori
Anahtar Kelimeler
azole antifungal drugs
MEPS-HPLC-DAD
method development
plasma and urine
sample preparation
Kurumlar
Houston Methodist
Houston United States
Interuniversity Consortium of Structural and Systems Biology
Rome Italy
Sapienza Università di Roma
Rome Italy
Selçuk Üniversitesi
Selçuklu Turkey
Università degli studi Magna Graecia di Catanzaro
Catanzaro Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
40
Atıf
10
Yazar
5
Anahtar Kelime