Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Traditionally used lathyrus species: Phytochemical composition, antioxidant activity, enzyme inhibitory properties, cytotoxic effects, and In Silico studies of L. Czeczottianus and L. Nissolia
Frontiers in Pharmacology · Şubat 2017
YÖKSİS Kayıtları
Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L-czeczottianus and L-nissolia
FRONTIERS IN PHARMACOLOGY · 2017 SCI
PROFESÖR GÖKHAN ZENGİN →
Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L. czeczottianus and L. nissolia
Frontiers in Pharmacology · 2017 SCI-Expanded
PROFESÖR GÖKHAN ZENGİN →
Traditionally Used Lathyrus Species: Phytochemical Composition, Antioxidant Activity, Enzyme Inhibitory Properties, Cytotoxic Effects, and in silico Studies of L. czeczottianus and L. nissolia
Frontiers in Pharmacology · 2017 SCI-Expanded
PROFESÖR ABDURRAHMAN AKTÜMSEK →
Makale Bilgileri
DergiFrontiers in Pharmacology
Yayın TarihiŞubat 2017
Cilt / Sayfa8
Scopus ID2-s2.0-85014192575
Erişim🔓 Açık Erişim
Özet
Members of the genus Lathyrus are used as food and as traditional medicines. In order to find new sources of biologically-active compounds, chemical and biological profiles of two Lathyrus species (L. czeczottianus and L. nissolia) were investigated. Chemical profiles were evaluated by HPLC-ESI-MSn, as well as by their total phenolic and flavonoid contents. In addition, antioxidant, enzyme inhibitory, and cytotoxic effects were also investigated. Antioxidant properties were tested by using different assays (DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation). Cholinesterases (AChE and BChE), tyrosinase, a-amylase, and a-glucosidase were used to evaluate enzyme inhibitory effects. Moreover, vitexin (apigenin-8-C-glucoside) and 5-O-caffeoylquinic acid were further subjected to molecular docking experiments to provide insights about their interactions at molecular level with the tested enzymes. In vitro cytotoxic effects were examined against human embryonic kidney cells (HEK293) by using iCELLigence real time cell analysis system. Generally, L. czeczottianus exhibited stronger antioxidant properties than L. nissolia. However, L. nissolia had remarkable enzyme inhibitory effects against cholinesterase, amylase and glucosidase. HPLC-ESI-MSn analysis revealed that flavonoids were major components in these extracts. On the basis of these results, Lathyrus extracts were rich in biologically active components; thus, these species could be utilized to design new phytopharmaceutical and nutraceutical formulations.
Yazarlar (11)
1
E. J. Llorent-Martínez
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Maria Luisa Fernández De Córdova
4
Onur Bender
ORCID: 0000-0003-0691-3508
5
Arzu Atalay
ORCID: 0000-0003-1309-5291
6
Ramazan Ceylan
7
Adriano Mollica
8
Andrei Mocan
9
Sengul Uysal
10
Gokalp Ozmen Guler
11
Abdurrahman Aktumsek
Anahtar Kelimeler
Antioxidant
Bioactive formulations
Cytotoxic
Flavonoid
Lathyrus
Molecular docking
Kurumlar
Ankara Üniversitesi
Ankara Turkey
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Castilla-La Mancha
Ciudad Real Spain
Universidad de Jaén
Jaen Spain
Universitatea de Medicina si Farmacie Iuliu Hatieganu din Cluj-Napoca
Cluj Napoca Romania
Universitatea de Stiinte Agricole si Medicina Veterinara din Cluj-Napoca
Cluj Napoca Romania
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
62
Atıf
11
Yazar
6
Anahtar Kelime