Scopus
🔓 Açık Erişim
Graminex pollen: Phenolic pattern, colorimetric analysis and protective effects in immortalized prostate cells (PC3) and rat prostate challenged with LPS
Molecules · Ocak 2018
Makale Bilgileri
DergiMolecules
Yayın TarihiOcak 2018
Cilt / Sayfa23
Scopus ID2-s2.0-85047193818
Erişim🔓 Açık Erişim
Özet
Prostatitis, a general term describing prostate inflammation, is a common disease that could be sustained by bacterial or non-bacterial infectious agents. The efficacy of herbal extracts with antioxidant and anti-inflammatory effects for blunting the burden of inflammation and oxidative stress, with possible improvements in clinical symptoms, is under investigation. Pollen extracts have been previously reported as promising agents in managing clinical symptoms related to prostatitis. The aim of the present work was to evaluate the protective effects of Graminex pollen (GraminexTM, Deshler, OH, USA), a commercially available product based on standardized pollen extracts, in rat prostate specimens, ex vivo. In this context, we studied the putative mechanism of action of pollen on multiple inflammatory pathways, including the reduction of prostaglandin E2 (PGE2), nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB), and malondialdehyde (MDA), whose activities were significantly increased by inflammatory stimuli. We characterized by means of chromatographic and colorimetric studies the composition of Graminex pollen to better correlate the activity of pollen on immortalized prostate cells (PC3), and in rat prostate specimens challenged with Escherichia coli lipopolysaccharide (LPS). We found that Graminex pollen was able to reduce radical oxygen species (ROS) production by PC3 cells and MDA, NFκB mRNA, and PGE2 levels, in rat prostate specimens. According to our experimental evidence, Graminex pollen appears to be a promising natural product for the management of the inflammatory components in the prostate.
Yazarlar (13)
1
Marcello Locatelli
ORCID: 0000-0002-0840-825X
2
Nicola Macchione
3
Claudio Ferrante
4
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
5
Lucia Recinella
6
Simone Carradori
7
Gokhan Zengin
ORCID: 0000-0001-6548-7823
8
Stefania Cesa
ORCID: 0000-0002-8649-0017
9
Lidia Leporini
10
Sheila Leone
11
Luigi Brunetti
12
Luigi Menghini
ORCID: 0000-0002-7346-7395
13
Giustino Orlando
Anahtar Kelimeler
Colorimetric analysis
Inflammation
NFκB mRNA
PGE2
Phenolic pattern
Pollen
Kurumlar
Sapienza Università di Roma
Rome Italy
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Milano
Milan Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
54
Atıf
13
Yazar
6
Anahtar Kelime