Scopus
Chemical characterization with in vitro biological activities of Gypsophila species
Journal of Pharmaceutical and Biomedical Analysis · Haziran 2018
Makale Bilgileri
DergiJournal of Pharmaceutical and Biomedical Analysis
Yayın TarihiHaziran 2018
Cilt / Sayfa155 · 56-69
Scopus ID2-s2.0-85053510236
Özet
Methanol-aqueous extracts from the aerial parts of Gypsophila glomerata (GGE), G. trichotoma (GTE) and G. perfoliata (GPE) were investigated for antioxidant potential using different in vitro models, as well as for phenolic and flavonoid contents. The possible anti-cholinesterase, anti-tyrosinase, anti-amylase and anti-glucosidase activities were also tested. The flavonoid variability was analyzed using ultra high-performance liquid chromatography (UHPLC) coupled with hybrid quadrupole-Orbitrap high resolution mass spectrometry (HRMS). Eleven C-glycosyl flavones and 4 O-glycosyl flavonoids, including 2”-O-pentosyl-6-C-hexosyl-apigenin/methylluteolin, as well as their mono(di)-acetyl derivatives were found in GGE. Both GGE and GTE shared 2”-pentosyl-6-C-hexosyl-luteolin together with the common saponarin, homoorientin, orientin, isovitexin and vitexin, while di C-glycosyl flavones were evidenced only in GPE. The highest radical scavenging in both ABTS and DPPH assays was noted in GPE, as well as ferric and cupric reducing abilities. However, GTE had the strongest metal chelating activity (17.44 ± 0.51 mg EDTAE/g extract). GPE and GGE were more potent as acetylcholinesterases inhibitors witnessed by 2.09 ± 0.02 mg GALAE/g extract and 1.59 ± 0.09 mgGALAE/g extract, respectively. All flavonoids were found in G. glomerata for the first time. Therefore, further isolation and structural elucidation of newly described acetylated flavonoids are needed in order to determine their relevance in the beneficial properties of the plant.
Yazarlar (7)
1
Dimitrina Zheleva-Dimitrova
ORCID: 0000-0002-1952-9903
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Vessela Balabanova
ORCID: 0000-0002-9938-6542
4
Yulian Voynikov
5
Valentin Lozanov
6
Irina Lazarova
7
Reneta Gevrenova
ORCID: 0000-0002-1254-2419
Anahtar Kelimeler
Antioxidant
Enzyme inhibitor
Flavonoids
Gypsophila
Natural products
Kurumlar
Medical University of Sofia
Sofia Bulgaria
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
27
Atıf
7
Yazar
5
Anahtar Kelime