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Multiple pharmacological approaches on Fibigia eriocarpa extracts by in vitro and computational assays

Fundamental and Clinical Pharmacology · Ağustos 2018

Makale Bilgileri

DergiFundamental and Clinical Pharmacology
Yayın TarihiAğustos 2018
Cilt / Sayfa32 · 400-413
Erişim🔓 Açık Erişim
Özet The ethyl acetate, methanolic, and water extracts of Fibigia eriocarpa were assessed for a panoply of bioactivities. Total phenolic and flavonoid content were quantified as well as individual phenolic compounds by HPLC-DAD. The in vitro antioxidant and enzyme (acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase) inhibitory potential of the extracts were evaluated. In silico molecular docking was used to investigate possible interaction between dominant compounds and selected enzymes. Vanillin (303 μg/g extract), apigenin (270 μg/g extract), and kaempferol (180 μg/g extract) were the main compounds in the ethyl acetate extract, while the methanolic extract was characterized by the presence of vanillin, rutin, and apigenin (616, 616 and 252 μg/g extract, respectively). (+)-catechin (1422 μg/g extract) was the main compound in the water extracts. The ethyl acetate extract was found to be a superior source of antioxidant compounds and enzyme inhibitors against above-mentioned enzymes. Docking studies revealed that p-hydroxybenzoic and (+)-catechin have the best scores for tyrosinase, while kaempferol and apigenin showed the best binding pose for α-glucosidase, AChE, and BChE. Results amassed herein are the first report on the phytochemical and biological attributes of F. eriocarpa, which tend to validate the pharmacological uses of this plant as an alternative medicine.

Yazarlar (6)

1
Gokhan Zengin
ORCID: 0000-0001-6548-7823
2
Devina Lobine
3
Adriano Mollica
4
Marcello Locatelli
ORCID: 0000-0002-0840-825X
5
Simone Carradori
6
Mohamad Fawzi Mahomoodally

Anahtar Kelimeler

antioxidant bioactive compounds Fibigia molecular docking natural enzyme inhibitors

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius

Metrikler

10
Atıf
6
Yazar
5
Anahtar Kelime

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