Scopus
Development of novel techniques to extract phenolic compounds from Romanian cultivars of Prunus domestica L. and their biological properties
Food and Chemical Toxicology · Eylül 2018
Makale Bilgileri
DergiFood and Chemical Toxicology
Yayın TarihiEylül 2018
Cilt / Sayfa119 · 189-198
Scopus ID2-s2.0-85047005321
Özet
In the present work, fourteen cultivars of Prunus domestica were analyzed to investigate their phenolic pattern with the purpose of using the leaves as potential resources of bioactive compounds in the pharmaceutical and food industry. Microwave-assisted extraction (MAE), dispersive liquid–liquid microextraction and sugaring-out liquid–liquid extraction techniques were optimized in order to obtain an exhaustive multi-component panel of phenolic compounds. The best phenolic-enriched recovery was achieved using MAE in water:methanol (30:70), and this procedure was further applied for quantitative analysis of phenolic compounds in real samples. In order to prove the safeness of these extracts, the biological potential of the Prunus cultivars was tested by several in vitro antioxidant and enzyme inhibitory assays. Moreover, their cytotoxicity was evaluated on human gingival fibroblasts (HGFs), and in most of the cases the treatment with different concentrations of extracts didn't show cytotoxicity up to 500 μg/mL. Only ‘Carpatin’ and ‘Minerva’ cultivars, at 250 and 500 μg/mL, reduced partially cell viability of HGFs population. Noteworthy, Centenar cultivar was the most active for the α-glucosidase inhibition (6.77 mmolACAE/g extract), whereas Ialomița cultivar showed the best antityrosinase activity (23.07 mgKAE/g extract). Overall, leaves of P. domestica represent a rich alternative source of bioactive compounds.
Yazarlar (14)
1
Andrei Mocan
2
Alina Diuzheva
ORCID: 0000-0003-2090-1634
3
Simone Carradori
4
Vasil Andruch
5
Chiara Massafra
6
Cadmiel Moldovan
7
Cristian R. Sisea
8
Jacobus P. Petzer
9
Anél Petzer
10
Susi Zara
11
Guya Diletta Marconi
12
Gokhan Zengin
ORCID: 0000-0001-6548-7823
13
Gianina Crişan
14
Marcello Locatelli
ORCID: 0000-0002-0840-825X
Anahtar Kelimeler
Biological activities
DLLME
HPLC-PDA
MAE
Prunus domestica L.
SULLE
Kurumlar
North-West University
Potchefstroom South Africa
Pavol Jozef Šafárik University in Košice
Kosice Slovakia
Selçuk Üniversitesi
Selçuklu Turkey
Universitatea de Medicina si Farmacie Iuliu Hatieganu din Cluj-Napoca
Cluj Napoca Romania
Universitatea de Stiinte Agricole si Medicina Veterinara din Cluj-Napoca
Cluj Napoca Romania
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
39
Atıf
14
Yazar
6
Anahtar Kelime