Scopus
HPLC-DAD profiles and pharmacological insights of Onobrychis argyrea subsp isaurica extracts
Computational Biology and Chemistry · Ekim 2018
Makale Bilgileri
DergiComputational Biology and Chemistry
Yayın TarihiEkim 2018
Cilt / Sayfa76 · 256-263
Scopus ID2-s2.0-85050870592
Özet
Onobrychis argyrea Boiss. subsp. Isaurica (Fabaceae), endemic to the eastern Mediterranean region, is a poorly studied medicinal plant. This study sets out to investigate into antioxidant and inhibitory activities of O. argyrea extracts (ethyl acetate, methanol, and water) against key enzymes linked to diabetes (α-amylase, α-glucosidase), Alzheimer's disease (acetylcholinesterase, butyrylcholinesterase), and skin hyperpigmentation (tyrosinase). Phytochemical composition was determined by HPLC-DAD and in silico approach used to provide additional insight of the possible interaction of the identified phenolic compounds with the studied enzymes. The methanol extract showed potent inhibitory action against acetylcholinesterase (1.55 mg GALAE/g extract), tyrosinase (61.61 mg KAE/g extract), and glucosidase (20.17 mmol ACAE/g extract). The methanol extract of O. argyrea exhibited potent radical scavenging potential (126.51 mg TE/g extract for DPPH scavenging assay) and reducing capacities (311.36 and 200.70 mg TE/g extract, for CUPRAC and FRAP assays, respectively). Quercetin, apigenin, and benzoic acid were identified in significant amounts in the methanol extract of O. argyrea. Quercetin interacted with the catalytic pocket of glucosidase by establishing hydrogen bonds with Ser157, Ser241, Asp307, and π-π interactions with His280 and Tyr158. The observed inhibitory effects of O. argyrea extracts on the studied enzyme suggest that this plant could be a promising source of naturally occurring chemical compounds for the management of diabetes, Alzheimer's disease, skin hyperpigmentation disorders, as well as, oxidative stress-related complications.
Yazarlar (6)
1
Gokalp Ozmen Guler
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Firat Karadag
4
Adriano Mollica
5
Carene Marie Nancy Picot-Allain
6
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Acetylcholinesterase
Amylase
Computational approaches
Glucosidase
HPLC-DAD
Natural agentsin silico
Onobrychis argyrea
Phyto-Pharmaceutics
Kurumlar
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
Metrikler
9
Atıf
6
Yazar
8
Anahtar Kelime