Scopus
Comparative in vitro studies of the biological potential and chemical composition of stems, leaves and berries Aronia melanocarpa's extracts obtained by subcritical water extraction
Food and Chemical Toxicology · Kasım 2018
Makale Bilgileri
DergiFood and Chemical Toxicology
Yayın TarihiKasım 2018
Cilt / Sayfa121 · 458-466
Scopus ID2-s2.0-85053849364
Özet
Preparation of functional products as well as natural-based products requires non-toxic but effective extraction techniques. In this study, subcritical water extraction was used for the extraction of different aronia parts in order to explore their potential. Stems, leaves and berries of Aronia melanocarpa were extracted under the following conditions: temperature 130 °C; pressure 35 bar; time 20 min. The total phenols and flavonoid contents of the produced extracts were evaluated by conventional spectrophotometric methods. Additionally, the main phenolic compounds were also identified and quantified by high performance liquid chromatography with diode array detection (HPLC-DAD). The biological potential of the extracts was evaluated by determining their antioxidant (DPPH, ABTS and lipid peroxidation assays), antimicrobial, enzyme inhibitory (cholinesterase and elastase) and cytotoxic effects (HeLa, A-549, LS-174T, MRC-5 cell lines). The results indicate that leaves and berries extracts exhibited stronger antioxidant action when compared with stems. The strongest cholinesterase and elastase inhibitory activity was also found in berries extract. Similarly, the extracts obtained from leaves and berries showed considerable cytotoxic effects against tested cell lines. A moderate antimicrobial effects was observed too. Demonstrated biological potential of all three aronia parts can trace a new road map for developing newly designed functional products.
Yazarlar (8)
1
Aleksandra Cvetanović Kljakić
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Zoran Zeković
4
Jaroslava Švarc-Gajić
5
Slavica Ražić
6
Ana Damjanović
7
Pavle Mašković
8
Milan Mitić
Anahtar Kelimeler
Antimicrobial activity
Antioxidants
Aronia
Cytotoxicity
Enzyme inhibitors
HPLC
Subcritical water extraction
Kurumlar
Faculty of Pharmacy
Belgrade Serbia
Faculty of Technology
Belgrade Serbia
Institute of Oncology and Radiology of Serbia
Belgrade Serbia
Selçuk Üniversitesi
Selçuklu Turkey
University of Kragujevac
Kragujevac Serbia
University of Niš
Nis Serbia
Metrikler
56
Atıf
8
Yazar
7
Anahtar Kelime