Scopus
YÖKSİS Eşleşti
Chemical, biological and molecular modelling analyses to probe into the pharmacological potential of Antidesma madagascariense Lam.: A multifunctional agent for developing novel therapeutic formulations
Journal of Pharmaceutical and Biomedical Analysis · Kasım 2018
YÖKSİS Kayıtları
Chemical, biological and molecular modelling analyses to probe into the pharmacological potential of Antidesma madagascariense Lam.: A multifunctional agent for developing novel therapeutic formulations
Journal of Pharmaceutical and Biomedical Analysis · 2018 SCI
PROFESÖR GÖKHAN ZENGİN →
Makale Bilgileri
DergiJournal of Pharmaceutical and Biomedical Analysis
Yayın TarihiKasım 2018
Cilt / Sayfa161 · 425-435
Scopus ID2-s2.0-85053078950
Özet
Antidesma madagascariense Lam. (AM), an indigenous medicinal plant to the Mascarene Islands, is used for the treatment of several diseases. We endeavoured to validate its use via evaluating the kinetics of inhibition of crude aqueous extract (CAE) and crude methanol extract (CME) of AM against key metabolic enzymes (pancreatic lipase, cholesterol esterase [CEase], acetylcholinesterase [AChE], and urease). In vitro antiglycation, antioxidant, cytotoxicity using iCELLigence real time cell analysis system and WST-1 methods, were used. LC–ESI–MS/MS was employed to determine the phenolic composition of the extracts and interaction of selected compounds to the studied enzymes was determined using in silico docking. AChE was inhibited by the CME of AM and CEase by the CAE. Both extracts were active inhibitors of urease and pancreatic lipase. Hyperoside (271.97 μg/g extract), present in large amount in the CME, docked to the enzymatic pocket of urease and CEase. The extracts showed competitive and mixed inhibition of urease and pancreatic lipase, respectively. The antioxidant capacity of the CME (6.61 μg GAE/mg crude extract) was higher compared to CAE (2.20 μg GAE/mg crude extract). AM extracts were significantly (p < 0.05) less potent than aminoguanidine in preventing advanced glycation end products formation. Toxicological screening revealed that both extracts were non-toxic on HEK-293 cells. AM crude extracts at concentrations ranging from 78 to 312 μg/ml did not cause a visible change in cell morphology compared to control. This study supports the safe use of AM as a biomedicine for the management and/or treatment of common non-communicable diseases.
Yazarlar (7)
1
Mohamad Fawzi Mahomoodally
2
Arzu Atalay
ORCID: 0000-0003-1309-5291
3
Marie Carene Nancy Picot
4
Onur Bender
ORCID: 0000-0003-0691-3508
5
Evrim Celebi
6
Adriano Mollica
7
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
Acetylcholinesterase
Cholesterol esterase
In silico
Mauritius
Urease
xCELLigence
Kurumlar
Ankara Üniversitesi
Ankara Turkey
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
Metrikler
17
Atıf
7
Yazar
6
Anahtar Kelime