Scopus
🔓 Açık Erişim
A comparative study of the in vitro enzyme inhibitory and antioxidant activities of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret from Pakistan: New sources of natural products for public health problems
South African Journal of Botany · Ocak 2019
Makale Bilgileri
DergiSouth African Journal of Botany
Yayın TarihiOcak 2019
Cilt / Sayfa120 · 146-156
Scopus ID2-s2.0-85046167173
Erişim🔓 Açık Erişim
Özet
Infusions, decoctions and tinctures were prepared from flowers of Butea monosperma (Lam.) Taub. and Sesbania grandiflora (L.) Poiret and evaluated for in vitro inhibition of enzymes implicated on the onset of neurological diseases (acetylcholinesterase: AChE and butyrylcholinesterase: BuChE), diabetes (α-glucosidase and α-amylase), obesity (lipase) and skin hyperpigmentation (tyrosinase). Extracts were also appraised for radical scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, and for metal chelating activity on copper and iron ions. Samples were evaluated for their total contents in different phenolics groups by spectrophotometric methods, for phenolic profile by high performance liquid chromatography e diode array detection (HPLC-DAD) and for mineral contents by microwave plasma-atomic emission spectrometry (MP-AE). Regarding B. monosperma, the tincture allowed for a moderate inhibition of AChE, the decoction was able to inhibit α-glucosidase and no activity was observed towards BuChE, α-amylase or lipase. All extracts had a low or moderate inhibition towards tyrosinase, and significant RSA and metal chelating potential. As for S. grandiflora, only the decoction inhibited AChE, none of the extracts was able to inhibit BuChE, all samples inhibited α-glucosidase and infusions and decoctions had similar inhibitory properties towards α-amylase. None of the extracts was active against lipase, but all were able to inhibit tyrosinase. Extracts had also significant RSA, moderate copper chelation and decoctions had the capacity to chelate iron. The most abundant macroelements in both species were potassium and calcium, while iron was the prevalent microelement, especially in B. monosperma. Both species had significant levels of phenolic compounds, and the main components in decoctions and infusions of B. monosperma were syringic and salicylic acids, while the major compound identified in tinctures was the flavonoid luteolin-7-O-glucoside. In S. grandiflora the most abundant were chlorogenic and neochlorogenic acids and catechin hydrate. Molecular docking studies on the most abundant molecules in S. grandiflora, (+)-catechin, chlorogenic acid and neochlorogenic acid, indicate that these compounds are able to dock to α-glucosidase in a similar manner than acarbose. Our results suggest that flowers of both species are a promising source of high value-added compounds with enzyme inhibitory and antioxidant properties.
Yazarlar (16)
1
M. Baessa
2
Maria João Rodrigues
3
Catarina Pereira
ORCID: 0000-0002-1131-8773
4
T. Santos
5
Nuno R. Neng
6
José M.F. Nogueira
7
L. Barreira
8
J. Varela
9
H. Ahmed
10
S. Asif
11
S. A. Boukhari
12
W. K. Kayani
13
Khawaja Shafique Ahmad
14
Gokhan Zengin
ORCID: 0000-0001-6548-7823
15
Adriano Mollica
16
Luisa Custodio
Anahtar Kelimeler
Diabetes
Medicinal plants
Minerals
Neuroprotection
Oxidative stress
Traditional medicine
Kurumlar
COMSATS University Islamabad
Islamabad Pakistan
Faculdade de Ciências da Universidade de Lisboa
Lisbon Portugal
PMAS-Arid Agriculture University Rawalpindi
Rawalpindi Pakistan
Selçuk Üniversitesi
Selçuklu Turkey
Sveriges lantbruksuniversitet
Uppsala Sweden
Universidade do Algarve
Faro Portugal
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Poonch Rawalakot
Rawalakot Pakistan
Metrikler
21
Atıf
16
Yazar
6
Anahtar Kelime