Scopus
YÖKSİS Eşleşti
In vitro determination of the remineralizing potential and cytotoxicity of non-fluoride dental varnish containing bioactive glass, eggshell, and eggshell membrane
European Archives of Paediatric Dentistry · Nisan 2023
YÖKSİS Kayıtları
In vitro determination of the remineralizing potential and cytotoxicity of non-fluoride dental varnish containing bioactive glass, eggshell, and eggshell membrane
European Archives of Paediatric Dentistry · 2023 SCI-Expanded
PROFESÖR İLHAMİ ÇELİK →
In vitro determination of the remineralizing potential and cytotoxicity of non-fluoride dental varnish containing bioactive glass, eggshell, and eggshell membrane
EUROPEAN ARCHIVES OF PAEDIATRIC DENTISTRY · 2023 DENTISTRY, ORAL SURGERY & MEDICINE - ESCI
PROFESÖR ERCAN DURMUŞ →
In vitro determination of the remineralizing potential and cytotoxicity of non-fluoride dental varnish containing bioactive glass, eggshell, and eggshell membrane
European Archives of Paediatric Dentistry · 2023 ESCI
PROFESÖR HAYRİYE ESRA ÜLKER →
Makale Bilgileri
DergiEuropean Archives of Paediatric Dentistry
Yayın TarihiNisan 2023
Cilt / Sayfa24 · 229-239
Scopus ID2-s2.0-85146796129
Özet
Purpose: This study compares in vitro remineralization potential and cytotoxicity of fluoride-free varnish combinations containing bioactive glass, eggshell, and membrane powder and fluoride varnish formulations on artificial caries lesions. Methods: Artificial caries lesions were formed in two windows on third molars. One of the windows was coated with one of the following varnish formulations: FV (fluoride varnish), F-BAGV (fluoride and bioactive glass containing varnish), BAGV (bioactive glass containing varnish), EPV (eggshell powder containing varnish), EMP–EPV (eggshell membrane protein and eggshell powder containing varnish), STMP-EMP–EPV (sodium trimetaphosphate-treated eggshell membrane protein and eggshell powder containing varnish). The samples were remineralized, then investigated under scanning electron microscopy, and elemental analyses were performed by X-ray dispersive analysis (EDX). In addition, the traditional colorimetric tetrazolium-based reduction assay (XTT) and the modern impedance-based real-time cell analysis system (RTCA) were used to investigate their cytotoxicity. Results: The varnish applied area’s Ca/P ratio was lower than stoichiometric hydroxyapatite except for EPV (1.66) and STMP-EMP–EPV (1.67) groups. Undiluted extracts of all varnishes, 1:2 dilutions of FV and F-BAGV groups were cytotoxic in XTT assay. In RTCA, the normalised cell index of the EMP–EPV and STMP-EMP–EPV groups was higher than the control group. Conclusion: Bioactive glass, eggshell, eggshell membrane proteins and STMP-treated eggshell and eggshell membrane protein containing varnish have similar remineralizing effect to fluoride-containing varnish on demineralized enamel. Integrating biological or bioactive components instead of fluoride into the dental varnishes might reduce cytotoxicity.
Yazarlar (6)
1
Ercan Durmus
ORCID: 0000-0003-4851-4230
2
T. Kölüş
ORCID: 0000-0002-0840-7126
3
E. Çoban
4
H. Yalçınkaya
5
H. E. Ülker
6
Ilhami Çelik
Anahtar Kelimeler
Caries
Dental varnish
Eggshell membrane
Eggshell powder
Remineralization
Kurumlar
Karamanoğlu Mehmetbey Üniversitesi
Karaman Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Metrikler
1
Atıf
6
Yazar
5
Anahtar Kelime