Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Untargeted metabolomic profiling, multivariate analysis and biological evaluation of the true mangrove (Rhizophora mucronata lam.)
Antioxidants · Ekim 2019
YÖKSİS Kayıtları
Untargeted Metabolomic Profiling, Multivariate Analysis and Biological Evaluation of the True Mangrove (Rhizophora mucronata Lam.)
Antioxidants · 2019 SCI-Expanded
PROFESÖR AHMET UYSAL →
Untargeted Metabolomic Profiling, Multivariate Analysis and Biological Evaluation of the True Mangrove (Rhizophora mucronata Lam.)
Antioxidants · 2019 SCI-Expanded
PROFESÖR GÖKHAN ZENGİN →
Makale Bilgileri
DergiAntioxidants
Yayın TarihiEkim 2019
Cilt / Sayfa8
Scopus ID2-s2.0-85073728403
Erişim🔓 Açık Erişim
Özet
Currently, there is a renewed interest towards the development of plant-based pharmacophores. In this work, 16 extracts prepared from the leaves, twigs, roots and fruits of a hydro-halophyte, Rhizophora mucronata Lam. (Family: Rhizophoraceae), were studied for possible antioxidant activity and the phenolic profiles established. Thereafter, enzymatic inhibitory activities (α-amylase, α-glucosidase, tyrosinase, acetyl-(AChE), butyrylcholinesterase (BChE), lipase, and elastase) were assessed. The total phenolic, flavonoid, phenolic acid, tannin, flavanol and triterpenoid content were estimated using standard assays. An untargeted metabolomics-based approach, based on ultra-high-pressure liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS) followed by multivariate statistics, was then used to comprehensively profile and describe the phenolics present. UHPLC-QTOF-MS allowed for putatively annotating 104 phenolic acids, 103 flavonols, 94 flavones, 71 anthocyanins, 66 tyrosols, 29 lignans, 15 alkylphenols and 10 stilbenes in the extracts. Nine strains (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus aureus (MRSA), Salmonella enteritidis, Sarcina lutea, Proteus mirabilis, Bacillus cereus and Candida albicans) were then used to investigate the antimicrobial properties. The methanolic twig extract exhibited significant reducing potential towards Cu (II)/Cu (I) and Fe (III)/Fe (II) (1336.88 ± 15.70 and 710.18 ± 21.04 mg TE/g, respectively) and was the most potent DPPH radical scavenger (807.07 ± 6.83 mg TE/g). Additionally, the methanolic twig extract showed significant inhibition against most targeted enzymes. Anti-microbial results showed that all extracts were active against MRSA. Multivariate analysis demonstrated that the phenolic profile of ethyl acetate extracts and leaves were the two most discriminative parameters in terms of solvents and organs, respectively. The present findings indicated that R. mucronata may be further explored for the management/prevention of oxidative stress, neurodegenerative complications and hyperpigmentation.
Yazarlar (9)
1
Nabeelah Bibi Sadeer
ORCID: 0000-0002-0602-0059
2
Gabriele Rocchetti
3
Biancamaria Senizza
4
Domenico Montesano
ORCID: 0000-0002-8857-2091
5
Gokhan Zengin
ORCID: 0000-0001-6548-7823
6
Ahmet Uysal
7
Rajesh Jeewon
8
Luigi Lucini
ORCID: 0000-0002-5133-9464
9
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Antimicrobial
Antioxidant
Enzyme inhibitors
Hyperpigmentation
Oxidative stress
Phytochemicals
True mangrove
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Università Cattolica del Sacro Cuore, Campus di Piacenza e Cremona
Piacenza Italy
Università degli Studi di Perugia
Perugia Italy
University of Mauritius
Reduit Mauritius
Metrikler
29
Atıf
9
Yazar
7
Anahtar Kelime