Scopus
HPLC-MS/MS chemical characterization and biological properties of Origanum onites extracts: a recent insight
International Journal of Environmental Health Research · Kasım 2019
Makale Bilgileri
DergiInternational Journal of Environmental Health Research
Yayın TarihiKasım 2019
Cilt / Sayfa29 · 607-621
Scopus ID2-s2.0-85058987724
Özet
This study investigated into the phytochemical profile and biological properties of extracts (methanol and aqueous) of Origanum onites based on the antioxidant, enzyme inhibitory, and antibacterial activities. The aqueous extract exhibited higher antioxidant activities in the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS), ferric reducing antioxidant power, cupric reducing antioxidant capacity, phosphomolybdenum, and metal chelating assays, compared to the methanol extract. In contrast, the methanol extract was the most effective inhibitor of acetylcholinesterase, butyrylcholinesterase, tyrosinase, α-amylase, and α-glucosidase. The methanol extract also showed higher antibacterial activity with highest inhibition against Escherichia coli (MIC = 6.25 mg/mL). The total phenolic content was higher in the aqueous extract while the methanol extract possessed higher total flavonoid content. A total of 28 and 18 compounds (belonging to polyphenols, flavonoids, terpenoids, and ester classes) were identified from the methanol and water extracts, respectively. These findings suggest that O. onites could be helpful in the management of oxidative stress-associated diseases including diabetes and neurodegenerative complications. Abbreviations: ABTS: 2,2‘-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid; ACAE: acarbose equivalent; AChE: acetylcholinesterase; AD: Alzheimer‘s disease; BChE: butyrylcholinesterase; CUPRAC: cupric reducing antioxidant capacity; DPPH: 1,1-diphenyl-2-picrylhydrazyl; EDTAE: EDTA equivalent; FRAP: ferric reducing antioxidant power; GAE: gallic acid equivalent; GALAE: galatamine equivalent; HPLC: high performance liquid chromatography; KAE: kojic acid equivalent; RE: rutin equivalents; TE: trolox equivalent; TPC: total phenolic content; TFC: total flavonoid content.
Yazarlar (8)
1
Mohamad Fawzi Mahomoodally
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Mustafa Onur Aladag
4
Haluk Ozparlak
5
Alina Diuzheva
ORCID: 0000-0003-2090-1634
6
József Jekő
7
Zoltán Cziáky
8
Muhammad Zakariyyah Aumeeruddy
Anahtar Kelimeler
antioxidant
bio-active compounds
enzyme inhibition
HPLC-MS/MS
O. onites
Kurumlar
Pavol Jozef Šafárik University in Košice
Kosice Slovakia
Selçuk Üniversitesi
Selçuklu Turkey
University of Mauritius
Reduit Mauritius
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
16
Atıf
8
Yazar
5
Anahtar Kelime