Scopus
Multi-targeted potential of Pittosporum senacia Putt.: HPLC-ESI-MS<sup>n</sup> analysis, in silico docking, DNA protection, antimicrobial, enzyme inhibition, anti-cancer and apoptotic activity
Computational Biology and Chemistry · Aralık 2019
Makale Bilgileri
DergiComputational Biology and Chemistry
Yayın TarihiAralık 2019
Cilt / Sayfa83
Scopus ID2-s2.0-85071661076
Özet
Pittosporum senacia (PS) Putt. (Pittosporaceae), indigenous to the Mascarene Islands, is a common ingredient in traditional medicines. However, there is currently a dearth of studies to validate some of these traditional claims. Given the broad traditional uses of PS against several diseases, we aimed to provide a comprehensive insight into the biological and chemical profile of P. senacia. The antioxidant, enzyme inhibitory activity, anticancer, and phytochemical composition of the methanolic extract of P. senacia leaf extracts were studied. The possible interaction and binding mode of the most abundant phytochemicals were studied via in silico docking experiments on tyrosinase and α-glucosidase. The mechanism behind the cytotoxic property of P. senacia extract for MDA-MB-231 was also examined using different methods including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability test checking apoptosis-associated genes, and wound healing assays. Twenty-six compounds were identified, of which caffeoylquinic acid derivatives, ferulic acid derivative, cinnamoylquinic acid derivative and two other polyphenols (oleuropeine and isoramnetin glucoside) being abundant, have been tested using in silico studies, against α-glucosidase and tyrosinase. The extract (IC50 = 118.8 μg/ml) exhibited time and dose dependent anti-proliferative effect on human breast cancer cell line, MDA-MB-231. According to the expression profile of apoptosis inhibitors and apoptosis promoters genes, expression of Bax and Bak genes were significantly increased compared to Bcl-2 and Birc5 genes. Based on wound healing analysis, cell migration was inhibited after the application of the plant extract. The present findings suggested that PS might be a good candidate as sources of bioactive compounds for designing functional applications.
Yazarlar (9)
1
Mohamad Fawzi Mahomoodally
2
Carene Marie Nancy Picot-Allain
3
Muzzammil Hosenally
4
Aslı Ugurlu Bayarslan
ORCID: 0000-0003-2131-2823
5
Adriano Mollica
6
Azzurra Stefanucci
7
E. J. Llorent-Martínez
8
Mehmet Cengiz Baloglu
9
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
Antimicrobial
Apoptosis
Diabetes
Mauritius
Pittosporaceae
Kurumlar
Kastamonu University
Kastamonu Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Jaén
Jaen Spain
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
Metrikler
20
Atıf
9
Yazar
5
Anahtar Kelime