Scopus
Antibiotic-Potentiating Activity of Phanostenine Isolated from Cissampelos sympodialis Eichler
Chemistry and Biodiversity · Aralık 2019
Makale Bilgileri
DergiChemistry and Biodiversity
Yayın TarihiAralık 2019
Cilt / Sayfa16
Scopus ID2-s2.0-85074755642
Özet
Cissampelos sympodialis Eichler is well studied and investigated for its antiasthmatic properties, but there are no data in the literature describing antibacterial properties of alkaloids isolated from this botanical species. This work reports the isolation and characterization of phanostenine obtained from roots of C. sympodialis and describes for the first time its antimicrobial and antibiotic modulatory properties. Phanostenine was first isolated from Cissampelos sympodialis and its antibacterial activities were determined. Chemical structures of the alkaloid isolate were determined using spectroscopic and chemical analyses. Phanostenine was also tested for its antibacterial activity against standard strains and clinical isolates of Escherichia coli and Staphylococcus aureus. Minimal inhibitory concentration (MIC) was determined in a microdilution assay and for the evaluation of antibiotic resistance-modifying activity. MIC of the antibiotics was determined in the presence or absence of phanostenine at sub-inhibitory concentrations. The evaluation of antibacterial activity by microdilution assay showed activity for all strains with better values against S. aureus ATCC 12692 and E. coli 27 (787.69 mm). The evaluation of aminoglycoside antibiotic resistance-modifying activity showed reduction in the MIC of the aminoglycosides (amikacin, gentamicin and neomycin) when associated with phanostenine, MIC reduction of antibiotics ranging from 21 % to 80 %. The data demonstrated that phanostenine possesses a relevant ability to modify the antibiotic activity in vitro. We can suggest that phanostenine presents itself as a promising tool as an adjuvant for novel antibiotics formulations against bacterial resistance.
Yazarlar (13)
1
Walmir E.M. Cunha
2
Cicera J. Camilo
3
Carla de F. A. Nonato
4
Johnatan W.S. Mendes
5
Natália K.G. de Carvalho
6
Henrique Douglas Melo Coutinho
7
Irwin R.A. Menezes
8
Telma L.G. de Lemos
9
Raimundo Braz-Filho
10
Fabiola F.G. Rodrigues
11
Edinardo F.F. Matias
12
Gokhan Zengin
ORCID: 0000-0001-6548-7823
13
José Galberto Martins da Costa
Anahtar Kelimeler
antibacterial activity
Cissampelos sympodialis
modifying antibiotic activity
novel antibiotics
phanostenine
Kurumlar
Centro Universitário Leão Sampaio
Juazeiro Brazil
Selçuk Üniversitesi
Selçuklu Turkey
Universidade Estadual do Norte Fluminense
Campos dos Goitacazes Brazil
Universidade Federal do Ceará
Fortaleza Brazil
Universidade Regional do Cariri
Crato Brazil
Metrikler
4
Atıf
13
Yazar
5
Anahtar Kelime