Scopus
🔓 Açık Erişim
Assessment of the pharmacological properties and phytochemical profile of bruguiera gymnorhiza (L.) lam using in vitro studies, in silico docking, and multivariate analysis
Biomolecules · Mayıs 2020
Makale Bilgileri
DergiBiomolecules
Yayın TarihiMayıs 2020
Cilt / Sayfa10
Scopus ID2-s2.0-85084507680
Erişim🔓 Açık Erişim
Özet
Bruguiera gymnorhiza (L.) Lam. is claimed to effectively manage a number of ailments including diabetes and associated complications. Nonetheless, no attempt has been made to delineate its pharmacological propensities and phytochemical profile. This study was designed to appraise the antioxidant and enzymatic inhibitory properties relevant to the management of diabetes mellitus, obesity, and neurodegenerative and skin disorders. A combination of colorimetric assays and ultra-high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) were applied for the phytochemical screening of leaf, root, twig, and fruit extracts (methanol and ethyl acetate). In vitro antioxidant evaluations were via radical scavenging abilities (DPPH, ABTS), reducing potential (FRAP, CUPRAC), chelating power, and total antioxidant capacity (phosphomolybdenum). Seven key metabolic enzymes (α-amylase, α-glucosidase, tyrosinase, elastase, lipase, AChE, and BChE) were targeted to determine the inhibitory effects. Multivariate and in silico docking analysis were performed on collected data. Methanolic fruit extract yielded the highest total phenolic, tannin, and triterpenoid contents (174.18 ± 4.27 mg GAE/g, 176.24 ± 3.10 mg CE/g, 63.11 ± 3.27 mg OAE/g, respectively); significantly depressed tyrosinase, elastase, and α-amylase activities (155.35 ± 0.29 mg KAE/g, 4.56 ± 0.10 mg CAE/g, 1.00 ± 0.05 mmol ACAE/g, accordingly); and harboured the most potent antioxidant capacities with DPPH, CUPRAC, FRAP (492.62 ± 5.31, 961.46 ± 11.18, 552.49 ± 8.71 mg TE/g, respectively), and phosphomolybdenum (4.17 ± 0.31 mmol TE/g) assays. Multivariate analysis suggested that the type of solvents used influenced the biological activities more compared to plant parts. Docking analysis showed that azelaic acid binds with tyrosinase by Van der Waals and conventional hydrogen bonds. We anticipate that the present study may establish baseline data on this halophyte that could open new avenues for the development of biomedicine.
Yazarlar (9)
1
Nabeelah Bibi Sadeer
ORCID: 0000-0002-0602-0059
2
Kouadio Ibrahime Sinan
3
Zoltán Cziáky
4
József Jekő
5
Gokhan Zengin
ORCID: 0000-0001-6548-7823
6
Rajesh Jeewon
7
Hassan H. Abdallah
8
Kannan RR Rengasamy
9
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Antioxidants
Docking
Enzymatic effects
Mangrove
Mauritius
Multivariate analysis
Phytochemicals
Kurumlar
Konkuk University
Seoul South Korea
Salahaddin University-Erbil
Erbil Iraq
Selçuk Üniversitesi
Selçuklu Turkey
Ton-Duc-Thang University
Ho Chi Minh City Viet Nam
University of Mauritius
Reduit Mauritius
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
19
Atıf
9
Yazar
7
Anahtar Kelime