Scopus
🔓 Açık Erişim
Water extract from inflorescences of industrial hemp futura 75 variety as a source of anti-inflammatory, anti-proliferative and antimycotic agents: Results from in silico, in vitro and ex vivo studies
Antioxidants · Mayıs 2020
Makale Bilgileri
DergiAntioxidants
Yayın TarihiMayıs 2020
Cilt / Sayfa9
Scopus ID2-s2.0-85085895715
Erişim🔓 Açık Erişim
Özet
Industrial hemp (Cannabis sativa) is traditionally cultivated as a valuable source of fibers and nutrients. Multiple studies also demonstrated antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences. On the other side, only a few studies explored the potential pharmacological application of polar extracts from inflorescences. In the present study, we investigated the water extract from inflorescences of industrial hemp Futura 75 variety, from phytochemical and pharmacological point of view. The water extract was assayed for phenolic compound content, radical scavenger/reducing, chelating and anti-tyrosinase effects. Through an ex vivo model of toxicity induced by lipopolysaccharide (LPS) on isolated rat colon and liver, we explored the extract effects on serotonin, dopamine and kynurenine pathways and the production of prostaglandin (PG)E2. Anti-proliferative effects were also evaluated against human colon cancer HCT116 cell line. Additionally, antimycotic effects were investigated against Trichophyton rubrum, Trichophyton interdigitale, Microsporum gypseum. Finally, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted in order to predict the putative targets underlying the observed pharmacological and microbiological effects. Futura 75 water extract was able to blunt LPS-induced reduction of serotonin and increase of dopamine and kynurenine turnover, in rat colon. Additionally, the reduction of PGE2levels was observed in both colon and liver specimens, as well. The extract inhibited the HCT116 cell viability, the growth of T. rubrum and T. interdigitale and the activity of tyrosinase, in vitro, whereas in silico studies highlighting the inhibitions of cyclooxygenase-1 (induced by carvacrol), carbonic anhydrase IX (induced by chlorogenic acid and gallic acid) and lanosterol 14-α-demethylase (induced by rutin) further support the observed pharmacological and antimycotic e_ects. The present findings suggest female inflorescences from industrial hemp as high quality by-products, thus representing promising sources of nutraceuticals and cosmeceuticals against inflammatory and infectious diseases.
Yazarlar (21)
1
Giustino Orlando
2
Lucia Recinella
3
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
4
Luigi Brunetti
5
Sheila Leone
6
Simone Carradori
7
Simonetta Cristina Di Simone
ORCID: 0000-0001-5041-3848
8
Maria Chiara Ciferri
9
Gokhan Zengin
ORCID: 0000-0001-6548-7823
10
Gunes Ak
11
Hassan H. Abdullah
12
Estefanía Cordisco
13
Maximiliano Sortino
14
Laura Svetaz
15
Matteo Politi
ORCID: 0000-0002-1185-8569
16
Paola Angelini
ORCID: 0000-0002-6862-1079
17
Stefano Covino
18
Roberto Venanzoni
ORCID: 0000-0002-7768-0468
19
Stefania Cesa
ORCID: 0000-0002-8649-0017
20
Luigi Menghini
ORCID: 0000-0002-7346-7395
21
Claudio Ferrante
Anahtar Kelimeler
Antimycotic
Anti-proliferative
Dopamine
Futura 75
Kynurenine
Serotonin
Kurumlar
Salahaddin University-Erbil
Erbil Iraq
Sapienza Università di Roma
Rome Italy
School of Pharmaceutical Sciences, Universiti Sains Malaysia
Minden Malaysia
Selçuk Üniversitesi
Selçuklu Turkey
Universidad Nacional de Rosario
Rosario Argentina
Università degli Studi di Perugia
Perugia Italy
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
30
Atıf
21
Yazar
6
Anahtar Kelime