Scopus
🔓 Açık Erişim
Antioxidant and neuroprotective effects induced by cannabidiol and cannabigerol in rat CTX-TNA2 astrocytes and isolated cortexes
International Journal of Molecular Sciences · Mayıs 2020
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiMayıs 2020
Cilt / Sayfa21
Scopus ID2-s2.0-85085094535
Erişim🔓 Açık Erişim
Özet
Cannabidiol (CBD) and cannabigerol (CBG) are Cannabis sativa terpenophenols. Although CBD’s effectiveness against neurological diseases has already been demonstrated, nothing is known about CBG. Therefore, a comparison of the effects of these compounds was performed in two experimental models mimicking the oxidative stress and neurotoxicity occurring in neurological diseases. Rat astrocytes were exposed to hydrogen peroxide and cell viability, reactive oxygen species production and apoptosis occurrence were investigated. Cortexes were exposed to K+ 60 mM depolarizing stimulus and serotonin (5-HT) turnover, 3-hydroxykinurenine and kynurenic acid levels were measured. A proteomic analysis and bioinformatics and docking studies were performed. Both compounds exerted antioxidant effects in astrocytes and restored the cortex level of 5-HT depleted by neurotoxic stimuli, whereas sole CBD restored the basal levels of 3-hydroxykinurenine and kynurenic acid. CBG was less effective than CBD in restoring the levels of proteins involved in neurotransmitter exocytosis. Docking analyses predicted the inhibitory effects of these compounds towards the neurokinin B receptor. Conclusion: The results in the in vitro system suggest brain non-neuronal cells as a target in the treatment of oxidative conditions, whereas findings in the ex vivo system and docking analyses imply the potential roles of CBD and CBG as neuroprotective agents.
Yazarlar (15)
1
Viviana di Giacomo
2
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
3
Lucia Recinella
4
Giustino Orlando
5
Amelia Cataldi
6
Monica Rapino
7
Valentina Di Valerio
8
Maurizio Ronci
9
Sheila Leone
10
Luigi Brunetti
11
Luigi Menghini
ORCID: 0000-0002-7346-7395
12
Gokhan Zengin
ORCID: 0000-0001-6548-7823
13
Gunes Ak
14
Hassan H. Abdallah
15
Claudio Ferrante
Anahtar Kelimeler
Apoptosis
Cannabidiol
Cannabigerol
Docking
Exocytosis
Neuroprotection
Oxidative stress
Proteomic analysis
Serotonin
Kurumlar
Salahaddin University-Erbil
Erbil Iraq
School of Pharmaceutical Sciences, Universiti Sains Malaysia
Minden Malaysia
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
49
Atıf
15
Yazar
9
Anahtar Kelime