Scopus
🔓 Açık Erişim
Identification of chemical profiles and biological properties of rhizophora racemosa g. Mey. extracts obtained by different methods and solvents
Antioxidants · Haziran 2020
Makale Bilgileri
DergiAntioxidants
Yayın TarihiHaziran 2020
Cilt / Sayfa9 · 1-37
Scopus ID2-s2.0-85086705890
Erişim🔓 Açık Erişim
Özet
Mangrove forests exemplify a multifaceted ecosystem since they do not only play a crucial ecological role but also possess medicinal properties. Methanolic, ethyl acetate and aqueous leaf and bark extracts were prepared using homogenizer‐assisted extraction (HAE), infusion and maceration (with and without stirring). The different extracts were screened for phytochemical profiling and antioxidant capacities in terms of radical scavenging (DPPH, ABTS), reducing potential (CUPRAC, FRAP), total antioxidant capacity and chelating power. Additionally, R. racemosa was evaluated for its anti‐diabetic (α‐amylase, α‐glucosidase), anti‐tyrosinase and anti‐cholinesterase (AChE, BChE) activities. Additionally, antimycotic and antibacterial effects were investigated against Eescherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Listeria monocytogenes, Enterobacter cloacae, Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Aspergillus fumigatus, Aspergillus niger, Trichoderma viride, Penicillium funiculosum, Penicillium ludwigii and Penicillium verrucosum. Finally, based on phytochemical fingerprint, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted to predict the putative targets, namely tyrosinase, lanosterol‐14‐α‐demethylase and E. coli DNA gyrase, underlying the observed biopharmacological and microbiological effects. The methanolic leave and bark extracts (prepared by both HAE and maceration) abounded with phenolics, flavonoids, phenolic acids and flavonols. Results displayed that both methanolic leaf and bark extracts (prepared by HAE) exhibited thehighest radical scavenging, reducing potential and total antioxidant capacity. Furthermore, our findings showed that the highest enzymatic inhibitory activity recorded was with the tyrosinase enzyme. In this context, bioinformatics analysis predicted putative interactions between tyrosinase and multiple secondary metabolites including apigenin, luteolin, vitexin, isovitexin, procyanidin B, quercetin and methoxy‐trihydroxyflavone. The same compounds were also docked against lanosterol‐14α‐demethylase and E. Coli DNA gyrase, yielding affinities in the submicromolar– micromolar range that further support the observed anti‐microbial effects exerted by the extracts. In conclusion, extracts of R. racemosa may be considered as novel sources of phytoanti‐oxidants and enzyme inhibitors that can be exploited as future first‐line pharmacophores.
Yazarlar (20)
1
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
2
Kouadio Ibrahime Sinan
3
Gokhan Zengin
ORCID: 0000-0001-6548-7823
4
Mohamad Fawzi Mahomoodally
5
Nabeelah Bibi Sadeer
ORCID: 0000-0002-0602-0059
6
Ouattara Katinan Etienne
7
Zoltán Cziáky
8
József Jekő
9
Jasmina Glamocilja
10
Marina Soković
ORCID: 0000-0002-7381-756X
11
Lucia Recinella
12
Luigi Brunetti
13
Sheila Leone
14
Hassan H. Abdullah
15
Paola Angelini
ORCID: 0000-0002-6862-1079
16
Giancarlo Angeles Flores
ORCID: 0000-0001-6651-9794
17
Roberto Venanzoni
ORCID: 0000-0002-7768-0468
18
Luigi Menghini
ORCID: 0000-0002-7346-7395
19
Giustino Orlando
20
Claudio Ferrante
Anahtar Kelimeler
Anti‐microbial activity
Enzyme inhibition
Homogenizer‐assisted extraction
In silico studies
Mangrove
Phytoanti‐oxidants
Phytochemical
Kurumlar
Duy Tan University
Da Nang Viet Nam
Salahaddin University-Erbil
Erbil Iraq
School of Pharmaceutical Sciences, Universiti Sains Malaysia
Minden Malaysia
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Perugia
Perugia Italy
Université de Cocody-Abidjan
Abidjan Cote d'Ivoire
University of Belgrade
Belgrade Serbia
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
18
Atıf
20
Yazar
7
Anahtar Kelime