Scopus
🔓 Açık Erişim
Phytochemical composition and enzyme inhibition studies of Buxus papillosa C.K. Schneid
Processes · Temmuz 2020
Makale Bilgileri
DergiProcesses
Yayın TarihiTemmuz 2020
Cilt / Sayfa8
Scopus ID2-s2.0-85088434702
Erişim🔓 Açık Erişim
Özet
The current research work is an endeavor to study the chemical profiling and enzyme-inhibition potential of different polarity solvent (n-hexane, dichloromethane-DCM and methanol-MeOH) extracts from the aerial and stem parts of Buxus papillosa C.K. Schneid. All the extracts were analyzed for HPLC-PDA phenolic quantification, while both (aerial and stem) DCM extracts were studied for UHPLC-MS phytochemical composition. The inhibitory activity against the clinically important enzymes having crucial role in different pathologies like skin diseases (tyrosinase), inflammatory problems (lipoxygenase-LOX) and diabetes mellitus (ff-amylase) were studied using standard in vitro bioassays. The DCM extracts upon UHPLC-MS analysis conducted in both negative and positive ionization modes has led to the tentative identification of 52 important secondary metabolites. Most of these belonged to the alkaloid, flavonoid, phenolic and triterpenoid classes. The HPLC-PDA polyphenolic quantification identified the presence of 10 phenolic compounds. Catechin was present in significant amounts in aerial-MeOH (7.62 0.45 ±g/g extract) and aerial-DCM (2.39 0.51-±g/g extract) extracts. Similarly, higher amounts of epicatechin (2.76 0.32-±g/g extract) and p-hydroxybenzoic acid (1.06 0.21 ±g/g extract) were quantified in aerial-DCM and stem-MeOH extracts, respectively. Likewise, all the extracts exhibited moderate inhibition against all the tested enzymes. These findings explain the wide usage of this plant in folklore medicine and suggest that it could be further studied as an origin of novel bioactive phytocompounds and for the designing of new pharmaceuticals.
Yazarlar (8)
1
Hammad Saleem
2
Thet Thet Htar
3
Rakesh Naidu
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Marcello Locatelli
ORCID: 0000-0002-0840-825X
6
Angela Tartaglia
7
Syafiq Asnawi Zainal Abidin
8
Nafees Ahemad
ORCID: 0000-0002-1846-3236
Anahtar Kelimeler
Amylase
Antioxidant
Buxus papillosa
Lipoxygenase
Phenolic compounds
Tyrosinase
Kurumlar
Monash University
Melbourne Australia
Monash University Malaysia
Bandar Sunway Malaysia
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Veterinary and Animal Sciences, Lahore
Lahore Pakistan
Metrikler
4
Atıf
8
Yazar
6
Anahtar Kelime