Scopus
🔓 Açık Erişim
Dereplication and quantification of the ethanol extract of Miconia albicans (Melastomaceae) by HPLC-DAD-ESI-/MS/MS, and assessment of its anti-hyperalgesic and anti-inflammatory profiles in a mice arthritis-like model: Evidence for involvement of TNF-α, IL-1β and IL-6
Journal of Ethnopharmacology · Ağustos 2020
Makale Bilgileri
DergiJournal of Ethnopharmacology
Yayın TarihiAğustos 2020
Cilt / Sayfa258
Scopus ID2-s2.0-85084349298
Erişim🔓 Açık Erişim
Özet
Ethnopharmacological relevance: Miconia albicans (Sw) Triana (Melastomataceae), a medicinal plant widely used by practitioners of folk medicine in the northeast of Brazil, has been used to treat chronic inflammatory disorders, such as rheumatoid arthritis (RA) and other joint conditions. Oddly, there is little research on the species. Aim of the study: We aimed to evaluate the anti-arthritic and anti-inflammatory profile of the ethanolic leaf extract of M. albicans (EEMA), as well as to perform dereplication and quantification by HPLC-DAD-ESI-/MS/MS. Materials and methods: The compounds present in the extracts were identified by HPLC-DAD-ESI-MS/MS. The possible anti-inflammatory effect of EEMA (50 and 100 mg/kg, p.o) was evaluated using the pleurisy model induced by carrageenan and its action on IL-1β and TNF-α levels was also evaluated. The RA model was induced through the intra-articular injection of complete Freund's adjuvant (CFA). Results: HPLC-DAD-ESI-MS/MS analysis identified 23 compounds, with glycoside flavonoids mainly derived from quercetin, and rutin being the main compounds. EEMA significantly reduced (p < 0.001) leukocyte migration in the pleurisy model and reduced TNF-α and IL-1β levels in pleural lavage (p < 0.001). In the CFA animal model, EEMA significantly reduced the nociceptive and hyperalgesic behaviors demonstrated by the rearing test (p < 0.01 or p < 0.05) and decreased mechanical hyperalgesia (p < 0.001). EEMA produced a significant improvement in mobility in the open-field test (only at the higher dose, p < 0.05). EEMA significantly (p < 0.01) increased hindpaw grip strength. The diameter of CFA-induced ipsilateral knee edema was significantly reduced (p < 0.001) by EEMA, which was related to reduced levels of IL-6 and TNF-α in the joint knee (p < 0.01). No indication of hepatic injury after chronic treatment was found. Conclusion: Taken together, these results contribute to the chemical and pharmacological knowledge of M. albicans and demonstrated that this medicinal plant appears to be able to mitigate deleterious symptoms of RA, which supports its use in folk medicine.
Yazarlar (17)
1
Lucindo J. Quintans-Júnior
2
Sathiyabama R. Gandhi
3
Fabiolla R.S. Passos
4
Luana Heimfarth
5
Erik W.Menezes Pereira
6
Brenda Souza Monteiro
7
Katielen Silvana dos Santos
8
Marcelo C. Duarte
9
Lucas Silva Abreu
10
Yuri M. Nascimento
ORCID: 0000-0002-9732-6191
11
Josean F. Tavares
12
Marcelo S. Silva
13
Irwin R.A. Menezes
14
Henrique Douglas Melo Coutinho
15
Adley A.N. Lima
16
Gokhan Zengin
ORCID: 0000-0001-6548-7823
17
Jullyana S.S. Quintans
Anahtar Kelimeler
Arthritis
Cytokine
Flavonoids
Inflammation
Medicinal plant
Pain
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Universidade Federal da Paraiba, Centro de Ciencias da Saude, Faculdade de Medicina
Joao Pessoa Brazil
Universidade Federal do Rio Grande do Norte
Natal Brazil
Universidade Regional do Cariri
Crato Brazil
Université Fédérale de Sergipe
Sao Cristovao Brazil
Metrikler
22
Atıf
17
Yazar
6
Anahtar Kelime