Scopus
🔓 Açık Erişim
Phytochemical Composition, Antioxidant Capacity, and Enzyme Inhibitory Activity in Callus, Somaclonal Variant, and Normal Green Shoot Tissues of Catharanthus roseus (L) G. Don
Molecules (Basel, Switzerland) · Ekim 2020
Makale Bilgileri
DergiMolecules (Basel, Switzerland)
Yayın TarihiEkim 2020
Cilt / Sayfa25
Scopus ID2-s2.0-85094826360
Erişim🔓 Açık Erişim
Özet
This study aimed to investigate the impact of plant growth regulators, sucrose concentration, and the number of subcultures on axillary shoot multiplication, in vitro flowering, and somaclonal variation and to assess the phytochemical composition, antioxidant capacity, and enzyme inhibitory potential of in vitro-established callus, somaclonal variant, and normal green shoots of Catharanthus roseus. The highest shoot induction rate (95.8%) and highest number of shoots (23.6), with a mean length of 4.5 cm, were attained when the C. roseus nodal explants (0.6-1 cm in length) were cultivated in Murashige and Skoog (MS) medium with 2 µM thidiazuron, 1 µM 2-(1-naphthyl) acetic acid (NAA), and 4% sucrose. The in vitro flowering of C. roseus was affected by sucrose, and the number of subcultures had a significant effect on shoot multiplication and somaclonal variation. The highest levels of phenolics and flavonoids were found in normal green shoots, followed by those in somaclonal variant shoots and callus. The phytochemicals in C. roseus extracts were qualified using liquid chromatography-tandem mass spectrometry. A total of 39, 55, and 59 compounds were identified in the callus, somaclonal variant shoot, and normal green shoot tissues, respectively. The normal green shoot extracts exhibited the best free radical scavenging ability and reducing power activity. The strongest acetylcholinesterase inhibitory effects were found in the callus, with an IC50 of 0.65 mg/mL.
Yazarlar (9)
1
O. New Lee
2
Gunes Ak
3
Gokhan Zengin
ORCID: 0000-0001-6548-7823
4
Zoltán Cziáky
5
József Jekő
6
Kannan RR Rengasamy
7
Han Yong Park
8
Doo Hwan Kim
9
Iyyakkannu Sivanesan
ORCID: 0000-0001-7447-6392
Anahtar Kelimeler
alkaloids
antioxidant activity
in vitro flowering
micropropagation
phenolics
somaclonal variation
Kurumlar
Konkuk University
Seoul South Korea
North-West University
Potchefstroom South Africa
Sejong University
Seoul South Korea
Selçuk Üniversitesi
Selçuklu Turkey
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
12
Atıf
9
Yazar
6
Anahtar Kelime