Scopus
Multidirectional insights into the phytochemical, biological, and multivariate analysis of the famine food plant (Calligonum polygonoides L).: A novel source of bioactive phytocompounds
Food Research International · Kasım 2020
Makale Bilgileri
DergiFood Research International
Yayın TarihiKasım 2020
Cilt / Sayfa137
Scopus ID2-s2.0-85088984705
Özet
Calligonum polygonoides L. also known as famine food plant, is normally consumed in times of food scarcity in India and Pakistan and also used traditionally in the management of common diseases. The present design aims to provide an insight into the medicinal potential of four solvent extracts of C. polygonoides via an assessment of its phytochemical profile, antioxidant and enzyme inhibitory potential. Phytochemical composition was estimated by deducing total bioactive constituents, UHPLC-MS secondary metabolites profile, and HPLC phenolic quantification. Antioxidant potential was determined via six methods (radical scavenging (DPPH and ABTS), reducing power (FRAP and CUPRAC), phosphomolybdenum total antioxidant capacity and metal chelation activity). Enzyme inhibitory potential was assessed against clinical enzymes (acetylcholinesterase -AChE, butyrylcholinesterase -BChE, tyrosinase, and α-amylase). The highest amounts of phenolic contents were found in chloroform extract (76.59 mg GAE/g extract) which may be attributed to its higher radical scavenging, reducing power and tyrosinase inhibition potential. The n-butanol extract containing the maximum amount of flavonoids (55.84 mg RE/g extract) exhibited highest metal chelating capacity. Similarly, the n-hexane extract was found to be most active against AChE (4.65 mg GALAE/g extract), BChE (6.59 mg GALAE/g extract), and α-amylase (0.70 mmol ACAE/g extract) enzymes. Secondary metabolite assessment of the crude methanol extract as determined by UHPLC-MS analysis revealed the presence of 24 (negative ionization mode) and 15 (positive ionization mode) secondary metabolites, with most of them belonging to phenolic, flavonoids, terpene, and alkaloid groups. Moreover, gallic acid and naringenin were the main phenolics quantified by HPLC-PDA analysis in all the tested extracts (except n-butanol extract). PCA statistical analysis was also conducted to establish any possible relationship amongst bioactive contents and biological activities. Overall, the C. polygonoides extracts could be further considered to isolate bioactive enzyme inhibitory and antioxidant natural phytocompounds.
Yazarlar (12)
1
Irfan Pervaiz
2
Hammad Saleem
3
Muhammad Sarfraz
4
Muhammad Imran Tousif
5
Umair Khurshid
6
Saeed Ahmad
7
Gokhan Zengin
ORCID: 0000-0001-6548-7823
8
Kouadio Ibrahime Sinan
9
Marcello Locatelli
ORCID: 0000-0002-0840-825X
10
Mohamad Fawzi Mahomoodally
11
Syafiq Asnawi Zainal Abidin
12
Nafees Ahemad
ORCID: 0000-0002-1846-3236
Anahtar Kelimeler
Antioxidants
Bioactive agents
Calligonum polygonoides
Enzyme inhibition
Phytochemicals
Kurumlar
Al Ain University
Al Ain United Arab Emirates
Duy Tan University
Da Nang Viet Nam
Monash University Malaysia
Bandar Sunway Malaysia
Selçuk Üniversitesi
Selçuklu Turkey
The Islamia University of Bahawalpur
Bahawalpur Pakistan
University of Education
Lahore Pakistan
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Lahore
Lahore Pakistan
University of Mauritius
Reduit Mauritius
University of Veterinary and Animal Sciences, Lahore
Lahore Pakistan
Metrikler
13
Atıf
12
Yazar
5
Anahtar Kelime