Scopus
🔓 Açık Erişim
Untargeted phytochemical profile, antioxidant capacity and enzyme inhibitory activity of cultivated and wild lupin seeds from tunisia
Molecules · Haziran 2021
Makale Bilgileri
DergiMolecules
Yayın TarihiHaziran 2021
Cilt / Sayfa26
Scopus ID2-s2.0-85108203719
Erişim🔓 Açık Erişim
Özet
Lupin seeds can represent a valuable source of phenolics and other antioxidant compounds. In this work, a comprehensive analysis of the phytochemical profile was performed on seeds from three Lupinus species, including one cultivar (Lupinus albus) and two wild accessions (Lupinus cossentinii and Lupinus luteus), collected from the northern region of Tunisia. Untargeted metabolomic profiling allowed to identify 249 compounds, with a great abundance of phenolics and alkaloids. In this regard, the species L. cossentinii showed the highest phenolic content, being 6.54 mg/g DW, followed by L. luteus (1.60 mg/g DW) and L. albus (1.14 mg/g DW). The in vitro antioxidant capacity measured by the ABTS assay on seed extracts ranged from 4.67 to 17.58 mg trolox equivalents (TE)/g, recording the highest values for L. albus and the lowest for L. luteus. The DPPH radical scavenging activity ranged from 0.39 to 3.50 mg TE/g. FRAP values varied between 4.11 and 5.75 mg TE/g. CUPRAC values for lupin seeds ranged from 7.20 to 8.95 mg TE/g, recording the highest for L. cossentinii. The results of phosphomolybdenum assay and metal chelation showed similarity between the three species of Lupinus. The acetylcholinesterase (AChE) inhibition activity was detected in each methanolic extract analyzed with similar results. Regarding the butyrylcholinesterase (BChE) enzyme, it was weakly inhibited by the Lupinus extracts; in particular, the highest activity values were recorded for L. albus (1.74 mg GALAE/g). Overall, our results showed that L. cossentinii was the most abundant source of polyphenols, consisting mainly in tyrosol equivalents (5.82 mg/g DW). Finally, significant correlations were outlined between the phenolic compounds and the in vitro biological activity measured, particularly when considering flavones, phenolic acids and lower-molecular-weight phenolics.
Yazarlar (10)
1
Amna Ben Hassine
2
Gabriele Rocchetti
3
Leilei Zhang
ORCID: 0000-0002-9278-0459
4
Biancamaria Senizza
5
Gokhan Zengin
ORCID: 0000-0001-6548-7823
6
Mohamad Fawzi Mahomoodally
7
Mossadok Ben-Attia
8
Youssef Rouphael
9
Luigi Lucini
ORCID: 0000-0002-5133-9464
10
Safia El-Bok
Anahtar Kelimeler
Acetylcholine esterase inhibition
Bioactive compounds
Foodomics
Functional components
Lupinus
Radical scavenging
UHPLC-QTOF-MS
Untargeted profiling
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Università Cattolica del Sacro Cuore, Campus di Piacenza e Cremona
Piacenza Italy
Università degli Studi di Napoli Federico II
Naples Italy
Université de Tunis El Manar, Faculté des Sciences de Tunis
Tunis Tunisia
University of Carthage
Tunis Tunisia
University of Mauritius
Reduit Mauritius
Metrikler
12
Atıf
10
Yazar
8
Anahtar Kelime