Scopus
🔓 Açık Erişim
LC-ESI-QTOF-MS/MS analysis, cytotoxic, antiviral, antioxidant and enzyme inhibitory properties of four extracts of Geranium pyrenaicum burm. F.: A good gift from the natural treasure
International Journal of Molecular Sciences · Temmuz 2021
Makale Bilgileri
DergiInternational Journal of Molecular Sciences
Yayın TarihiTemmuz 2021
Cilt / Sayfa22
Scopus ID2-s2.0-85110212461
Erişim🔓 Açık Erişim
Özet
This study focused on the biological evaluation and chemical characterization of Geranium pyrenaicum Burm. f. Different solvent extracts (hexane, ethyl acetate, methanol, and water extracts) were prepared. The phytochemical profile, antioxidant, and enzyme inhibitory activity were investigated. Cytotoxicity was assessed using VERO, FaDu, HeLa and RKO cells. The antiviral activity was carried out against HSV-1 (Herpes simplex virus 1) propagated in VERO cell line. The aqueous extract, possessing high phenolic content (170.50 mg gallic acid equivalent/g extract), showed the highest reducing capacity (613.27 and 364.10 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively), radical scavenging potential (469.82 mg Trolox equivalent/g extract, against 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), metal chelating ability (52.39 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant capacity (3.15 mmol Trolox equivalent/g extract). Liquid chromatographyelectrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) alloved to tentatively identify a total of 56 compounds in the extracts, including ellagitannins, gallic acid and galloyl derivatives amongst others. The ethyl acetate extracts substantially depressed cholinesterase enzymes (4.49 and 12.26 mg galantamine equivalent/g extract against AChE and BChE, respectively) and α-amylase enzyme (1.04 mmol acarbose equivalent/g extract). On the other hand, the methanolic extract inhibited tyrosinase (121.42 mg kojic acid equivalent/g extract) and α-glucosidase (2.39 mmol acarbose equivalent/g extract) activities. The highest selectivity towards all cancer cell lines (SI 4.5–10.8) was observed with aqueous extract with the FaDu cells being the most sensitive (CC50 40.22 μg/mL). It can be concluded that the presence of certain bioactive antiviral molecules may be related to the high anti HSV-1 activity of the methanolic extract. This work has generated vital scientific data on this medicinal plant, which is a prospective candidate for the creation of innovative phyto-pharmaceuticals.
Yazarlar (11)
1
Łukasz Świątek
ORCID: 0000-0003-1626-680X
2
Elwira Sieniawska
ORCID: 0000-0003-4773-0329
3
Kouadio Ibrahime Sinan
4
Magdalena Maciejewska-Turska
ORCID: 0000-0003-4113-9079
5
Anastazja Boguszewska
6
Małgorzata Polz-Dacewicz
ORCID: 0000-0002-3222-184X
7
Ismail Senkardes
8
Gokalp Ozmen Guler
9
Nabeelah Bibi Sadeer
ORCID: 0000-0002-0602-0059
10
Mohamad Fawzi Mahomoodally
11
Gokhan Zengin
ORCID: 0000-0001-6548-7823
Anahtar Kelimeler
Antioxidants
Cytotoxicity
Enzymes
Geranium
HSV-1
Polyphenols
Kurumlar
Marmara Üniversitesi
Istanbul Turkey
Medical University of Lublin
Lublin Poland
Necmettin Erbakan Üniversitesi
Meram Turkey
Selçuk Üniversitesi
Selçuklu Turkey
University of Mauritius
Reduit Mauritius
Metrikler
21
Atıf
11
Yazar
6
Anahtar Kelime