Scopus
🔓 Açık Erişim
Shedding light into the connection between chemical components and biological effects of extracts from epilobium hirsutum: Is it a potent source of bioactive agents from natural treasure?
Antioxidants · Eylül 2021
Makale Bilgileri
DergiAntioxidants
Yayın TarihiEylül 2021
Cilt / Sayfa10
Scopus ID2-s2.0-85114006635
Erişim🔓 Açık Erişim
Özet
Epilobium hirsutum is extensively used as a traditional remedy in folk medicine, especially against prostate inflammation. Therefore, we evaluated the chemical profiles and biopharmaceutical potentials of different extracts of E. hirsutum aerial parts and roots. Metabolomic, antioxidant, and enzyme inhibitory profiles were investigated. Human prostate cancer PC3 cells were exposed to the extracts to evaluate antiproliferative effects. Gene expression and bioinformatics analyses were performed to investigate anti-inflammatory mechanisms. Oenothein B and myricetin were prominent compounds in the extracts. In scavenging/reducing assays, the methanol, infusion, and methanol/water extracts exhibited similar activities. We also observed the reduction of PC3 viability occurring following exposure to methanol and methanol/water extracts. According to bioinformatics analysis, myricetin was predicted to interact with COX-2 and TNFα. The interaction between TNFα and oxo-dihydroxy-octadecenoic acid was predicted as well. Intriguingly, the gene expression of COX-2 and TNFα was reduced in PC3 cells after exposure to methanol and methanol/water extracts. These effects were paralleled by the decreased gene expression of IL-8 and NFkB and the inhibition of PGE2 release. Therefore, the present findings suggest the potential use of E. hirsutum for the management of the burden of inflammation and oxidative stress occurring in lower urinary tract diseases, including prostatitis.
Yazarlar (12)
1
Gunes Ak
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Mohamad Fawzi Mahomoodally
4
E. J. Llorent-Martínez
5
Giustino Orlando
6
Annalisa Chiavaroli
ORCID: 0000-0002-3399-967X
7
Luigi Brunetti
8
Lucia Recinella
9
Sheila Leone
10
Simonetta Cristina Di Simone
ORCID: 0000-0001-5041-3848
11
Luigi Menghini
ORCID: 0000-0002-7346-7395
12
Claudio Ferrante
Anahtar Kelimeler
Antioxidants
Antiproliferative effects
Bioinformatics
Epilobium hirsutum
Gene expression
Myricetin
Oenothein B
Kurumlar
Selçuk Üniversitesi
Selçuklu Turkey
Universidad de Jaén
Jaen Spain
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
University of Mauritius
Reduit Mauritius
Metrikler
13
Atıf
12
Yazar
7
Anahtar Kelime