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Lc-ms based analysis and biological properties of pseudocedrela kotschyi (Schweinf.) harms extracts: A valuable source of antioxidant, antifungal, and antibacterial compounds

Antioxidants · Ekim 2021

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YÖKSİS Kayıtları
LC-MS Based Analysis and Biological Properties of Pseudocedrela kotschyi (Schweinf.) Harms Extracts: A Valuable Source of Antioxidant, Antifungal, and Antibacterial Compounds
Antioxidants · 2021 SCI-Expanded
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Makale Bilgileri

DergiAntioxidants
Yayın TarihiEkim 2021
Cilt / Sayfa10
Erişim🔓 Açık Erişim
Özet The impact of two extraction solvents on the phenolic composition, antioxidant, and enzymes inhibitory and antimicrobial activities of two parts (leaves and stem bark) of P. kotschyi was studied. Two different LC-DAD-MSn approaches were used to identify and quantify the bioac-tive compounds in the different extracts. A total of thirty-two compounds were quantified, being the procyanidin the most abundant in stem bark while catechin and flavonoids are most abundant in leaves. Overall, the stem bark extraction using methanol showed higher amounts of total phenolic (131.83 ± 1.81 mg GAE/g) and flavanol (14.14 ± 0.11 mg CE/g) while the leaves extraction using water exhibited stronger levels of total flavonoid (44.95 ± 0.38 mg RE/g) and phenolic acid (63.58 ± 2.00 mg CAE/g). As regards the antioxidant assays, methanol stem bark extracts were characterized by the highest antioxidant activities (DPPH: 1.94 ± 0.01 mmol TE/g, ABTS: 3.31 ± 0.01 mmol TE/g, FRAP: 2.86 ± 0.02 mmol TE/g, CUPRAC: 5.09 ± 0.08 mmol TE/g, phospho-molybdenum: 5.16 ± 0.23 mmol TE/g and metal chelating: 17.12 ± 0.46 mg EDTAE/g). In addition, the methanolic extracts of stem bark had highest impact on acetylcholinesterase (2.54 mg GALAE/g), butyrylcholinesterase (5.48 mg GALAE/g). In contrast, the methanolic extracts of leaves was potent against tyrosinase (77.39 ± 0.21 mg KAE/g) and α-glucosidase (0.97 ± 0.01 mmol ACAE/g), while a higher anti-α–amylase (0.97 ± 0.01 mmol ACAE/g) was observed for water extracts of the same part. All of the tested extracts showed inhibitory effects on elastase, except methanolic leaves ex-tracts. Additionally, the extracts exhibited appreciable antifungal toward A. ochraceus, A. fumigatus, P. ochrochloron, T. viride, and P. funiculosum and promising antibacterial activity against M. flavus, S. aureus, L. monocytogenes, E. coli, P. aeruginosa, E. cloacae, and S. typhimurium. Taken together, the outcomes demonstrated P. kotschyi as a novel source of bioactive molecules of interest with an evident therapeutic value.

Yazarlar (10)

1
Kouadio Ibrahime Sinan
2
Stefano Dall'Acqua
3
Irene Ferrarese
4
Adriano Mollica
5
Azzurra Stefanucci
6
Jasmina Glamočlija
7
Marina Soković
ORCID: 0000-0002-7381-756X
8
Marija Nenadić
9
Abdurrahman Aktumsek
10
Gokhan Zengin
ORCID: 0000-0001-6548-7823

Anahtar Kelimeler

Antimicrobial activity Antioxidants Enzyme inhibition LC-MS n Multi-block discriminant analysis Pseudocedrela kotschyi

Kurumlar

Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Padova
Padua Italy
University of Belgrade
Belgrade Serbia
University of G. d'Annunzio Chieti and Pescara
Chieti Italy

Metrikler

21
Atıf
10
Yazar
6
Anahtar Kelime

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