Scopus
🔓 Açık Erişim
Phytochemical constituents and biological activities of the unexplored plant rhinanthus angustifolius subsp. Grandiflorus
Applied Sciences (Switzerland) · Ekim 2021
Makale Bilgileri
DergiApplied Sciences (Switzerland)
Yayın TarihiEkim 2021
Cilt / Sayfa11
Scopus ID2-s2.0-85116386203
Erişim🔓 Açık Erişim
Özet
In the present study, a total of 12 extracts of Rhinanthus angustifolius subsp. grandiflorus, an understudied hemiparasitic species, were obtained using different extraction techniques, namely, homogenizer-assisted extraction (HAE), maceration (MAC), soxhlet (SOX), infusion, and solvents (ethyl acetate, methanol, ethanol, and water), and were evaluated for their in vitro antioxidant and enzyme-inhibiting properties. Additionally, untargeted profiling based on high-resolution mass spec-trometry targeted different phytochemical classes, namely, polyphenols, terpenoids, and alkaloids. The highest total phenolic and flavonoid contents were detected using methanol as the extraction sol-vent. Multivariate statistics following the untargeted profiling revealed that the extraction solvent had a hierarchically higher impact than the extraction method when considering the recovery of bioactive compounds. The methanolic extracts displayed the highest radical-scavenging antioxidant capacity, as provided by CUPRAC and FRAP assays. On the other hand, the water extracts (MAC and HAE) and the infusion extract showed the highest activity as metal chelators (25.66–27.51 mg EDTAE/g). Similarly, the water extract obtained by HAE and the infusion extract revealed the highest phospho-molybdenum activity (3.92 ± 0.14 and 3.71 ± 0.01 mmol TE/g, respectively). The different extracts also exhibited different enzyme inhibition potentials. For instance, HAE and MAC ethanolic extracts inhibited only α-amylase (0.69 ± 0.01 and 0.70 ± 0.01 mmol ACAE/g), while all the other extracts showed a dual inhibition against both carbohydrate-hydrolyzing enzymes tested (i.e., α-amylase: 0.07–0.69 mmol ACAE/g; α-glucosidase: 0.03–1.30 mmol ACAE/g). Nevertheless, the other extracts inhibited acetyl-, butyryl-cholinesterases, or both; MAC–water extract displayed no inhibition against the enzymes. Additionally, all the studied extracts were found to inhibit tyrosinase, ranging from 10.62 to 52.80 mg KAE/g. In general, the water extracts showed weaker inhibition towards the enzymes than the other extracts. This study demonstrated that R. angustifolius is an excellent source of natural antioxidants and enzyme inhibitors that could be further investigated and exploited for pharmaceutical purposes.
Yazarlar (9)
1
Leilei Zhang
ORCID: 0000-0002-9278-0459
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Gabriele Rocchetti
4
Ismail Senkardes
5
Sharmeen Jugreet
6
Mohamad Fawzi Mahomoodally
7
Tapan Behl
8
Youssef Rouphael
9
Luigi Lucini
ORCID: 0000-0002-5133-9464
Anahtar Kelimeler
Antioxidants
Enzyme inhibitors
Metabolomics
Phenolics/flavonoids
Rhinanthus angustifolius
Solvent extraction
UHPLC-QTOF-MS
Kurumlar
Chitkara University, Punjab
Rajpura India
Marmara Üniversitesi
Istanbul Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Università Cattolica del Sacro Cuore, Campus di Piacenza e Cremona
Piacenza Italy
Università degli Studi di Napoli Federico II
Naples Italy
University of Mauritius
Reduit Mauritius
Metrikler
5
Atıf
9
Yazar
7
Anahtar Kelime