Scopus
🔓 Açık Erişim
Antioxidant activity of stryphnodendron rotundifolium mart. Stem bark fraction in an iron overload model
Foods · Kasım 2021
Makale Bilgileri
DergiFoods
Yayın TarihiKasım 2021
Cilt / Sayfa10
Scopus ID2-s2.0-85118770235
Erişim🔓 Açık Erişim
Özet
Stryphnodendron rotundifolium Mart., popularly known as “barbatimão”, is a plant species traditionally used by topical and oral routes for the treatment of infectious and inflammatory diseases. Considering the well-described antioxidant properties of this species, this study investigated the protective effects of its keto-aqueous extract using an in vitro model of iron overload. Phenolic compounds were quantified and identified by Ultra-Performance Liquid Chromatography coupled with quadrupole Time-Of-Flight Electrospray Ionization Mass Spectrometry (UPLC–ESI-qTOF-MS/MS) in positive and negative ions mode analysis. Antioxidant activity was analyzed following the iron-chelating–reducing capacity and deoxyribose degradation (2-DR) protection methods. The analysis identified condensed tannins (54.8 mg catechin/g dry fraction (DF), polyphenols (25 mg gallic acid/g DF), and hydrolyzable tannins (28.8 mg tannic acid/g DF). Among the constituents, prodelphinidin, procyanidin, and prorobinetinidine were isolated and identified. The extract sig-nificantly protected 2-DR degradation induced by Fe2+ (72% protection) or• OH (43% protection). The ortho-phenanthroline test revealed Fe2+-chelating and Fe3+-reducing activities of 93% and 84%, respectively. A preliminary toxicological analysis using Artemia salina revealed mortality below 10%, at a concentration of 0.25 mg/mL, indicating low toxicity under the present experimental conditions. In conclusion, the findings of the present study indicate that Stryphnodendron rotundifolium is a source of antioxidant compounds with the potential to be used in drug development in the context of iron overload disorders, which remains to be further investigated in vivo.
Yazarlar (12)
1
Gerson Javier Torres Salazar
2
Francisco Junio Dias
3
Paulo Riceli Vasconcelos Ribeiro
4
Edy Sousa de Brito
5
Kirley Marques Canuto
6
Henrique Douglas Melo Coutinho
7
Jaime Ribeiro-Filho
8
Monica Gallo
9
Domenico Montesano
ORCID: 0000-0002-8857-2091
10
Daniele Naviglio
ORCID: 0000-0001-5191-7669
11
Gokhan Zengin
ORCID: 0000-0001-6548-7823
12
José Galberto Martins da Costa
Anahtar Kelimeler
Antioxidant activity
Free radicals
Iron overload
Stryphnodendron rotundifolium
Tannins
UPLC–ESI-qTOF-MS/MS
Kurumlar
Embrapa Agroindústria Tropical
Fortaleza Brazil
Fundacao Oswaldo Cruz
Rio de Janeiro Brazil
Selçuk Üniversitesi
Selçuklu Turkey
Universidade Regional do Cariri
Crato Brazil
Università degli Studi di Napoli Federico II
Naples Italy
Metrikler
3
Atıf
12
Yazar
6
Anahtar Kelime