Scopus
Insight into the phytochemical composition, biological activities and docking studies of Moringa oleifera Lam. to authenticate its use in biopharmaceutical industries
Industrial Crops and Products · Kasım 2021
Makale Bilgileri
DergiIndustrial Crops and Products
Yayın TarihiKasım 2021
Cilt / Sayfa172
Scopus ID2-s2.0-85115055903
Özet
Moringa oleifera Lam. is a fast-growing deciduous tree native to the subcontinent with a wide array of uses. It is used as food plant by human and animals. Since it contains beneficial higher calcium, vitamin A, vitamin C, protein, potassium and iron contents, it is also used to treat malnutrition in children. The present study is conducted for comprehensive phytochemical profiling via total bioactive contents and ultra-high performance liquid chromatography (UHPLC-MS) analyses, and biological profiling by determining the antioxidant potential via DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), FRAP (ferric reducing antioxidant power), CUPRAC (cupric reducing antioxidant power), phosphomolybdenum and metal chelation assays. In addition, α-amylase, α-glucosidase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and tyrosinase inhibition assays were also performed. Multivariate statistical analysis was used to investigate the relationship between biological activities and total bioactive content of the extracts. Chemical profiling showed that total phenolic contents are present in highest concentrations in ethyl acetate extracts of the aerial parts and seed. Further total bioactive contents were correlated with their optimum anti-oxidant capacities for ABTS, FRAP and CUPRAC. Altogether 67 phytochemicals were identified from aerial parts of the plant and its seed with major classes as phenolics, flavonoids, sesquiterpenoids and alkaloids. The ethyl acetate extract of seed showed good inhibition of AChE (2.77 mg GALAE/g extract) and BChE (8.05 mg GALAE/g extract) followed by the inhibition potential of water extract as 8.34 mg GALAE/g extract. The ethyl acetate extract also showed most significant (61.93 mg-KAE/g) tyrosinase inhibitory activity. The hexane extract of seed showed good inhibition of α-glucosidase (6.32 mmol ACAE/g extract) and α-amylase (0.47 mmol ACAE/g extract). In silico docking studies were performed, where two herbimycin and prekinamycin showed good binding affinity with all the enzymes. It can be concluded that the presence of complex phytochemicals with multi-functional potential distinguishes Moringa oleifera Lam. as a promising source of health-promoting component and recommended its use in biopharmaceutical industries.
Yazarlar (12)
1
Jallat Khan
2
Muhammad Imran Tousif
3
Muhammad Saleem
4
Mamona Nazir
5
Saba Touseef
6
Kaynat Saleem
7
Sumreen Asim
8
Ahmad Khan
9
Muhammad Adnan Asghar
10
Gokhan Zengin
ORCID: 0000-0001-6548-7823
11
Nusrat Shafiq
ORCID: 0000-0002-3270-4227
12
Muther Mansoor Qaisrani
Anahtar Kelimeler
Antioxidant
Chemical profiling
Docking studies
Enzyme inhibition
Moringa oleifera Lam.
Multivariate statistical analysis
Kurumlar
Government College Women University Faisalabad
Faisalabad Pakistan
Khwaja Fareed University of Engineering & Information Technology
Rahim Yar Khan Pakistan
Quaid-i-Azam University
Islamabad Pakistan
Selçuk Üniversitesi
Selçuklu Turkey
The Government Sadiq College Women University Bahawalpur
Bahawalpur Pakistan
The Islamia University of Bahawalpur
Bahawalpur Pakistan
University of Education
Lahore Pakistan
University of Karachi
Karachi Pakistan
Metrikler
13
Atıf
12
Yazar
6
Anahtar Kelime