Scopus
🔓 Açık Erişim
Micropropagation, phytochemistry and biological activity of the critically endangered Mammillaria herrerae Werdermann
South African Journal of Botany · Aralık 2021
Makale Bilgileri
DergiSouth African Journal of Botany
Yayın TarihiAralık 2021
Cilt / Sayfa143 · 312-321
Scopus ID2-s2.0-85097365964
Erişim🔓 Açık Erişim
Özet
This study intended to develop an efficient in vitro micropropagation technique for the critically endangered ornamental cactus Mammillaria herrerae Werdermaan and to assess the phytochemical profile, antioxidant, and enzyme inhibitory activities of callus and shoot cultures. The greatest shoot induction (99.2 ± 0.8%), with more multiple shoots (15.4 ± 2.1), and maximum shoot length (0.83 ± 0.12) were achieved on nutrient medium Murashige and Skoog (MS) with N6-furfuryladenine (6-KN, 2.5 µM) and 3-indolebutyric acid (IBA, 1.0 µM). The maximum frequency of callus induction (100%) and callus growth (5.37 ± 0.22 FW g/100 mL) were obtained on a nutrient medium MS with 1-phenyl-3-(1,2,3,-thiadiazol-5-yl)urea (TDZ, 10.0 µM) and 2-(1-naphthyl) acetic acid (NAA, 5.0 µM). The highest number of roots (8.1 ± 1.6) and maximum mean length of root (4.2 ± 0.5) and shoot (1.4 ± 0.2 cm) were observed on a rooting medium containing IBA (0.5 µM). The in vitro-developed M. herrerae plantlets were acclimatized in the greenhouse with 92% survival. Shoots containing a higher number of phytochemicals than callus cultures. Thirty-five compounds were found in shoot extract and twenty-six compounds in callus extract. Also, the total flavonoid content of shoot (0.96 mg RE/g extract) was higher than callus (0.41 mg RE/g extract). The shoot extracts displayed the best metal chelating and acetylcholinesterase inhibitory activities. The protocol developed for M. herrerae could be utilized for bioactive compounds production, ex situ conservation, and commercial clonal propagation of this rare ornamental cactus.
Yazarlar (9)
1
Kihwan Song
2
Hyeonjeong Kang
3
Gunes Ak
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Zoltán Cziáky
6
József Jekő
7
Doo Hwan Kim
8
O. New Lee
9
Iyyakkannu Sivanesan
ORCID: 0000-0001-7447-6392
Anahtar Kelimeler
Antioxidant activity
Conservation
Cytokinin
Enzyme inhibition
Flavonoids
In vitro propagation
Phenolics
Kurumlar
Babo Orchid Farm
Namyangju-si South Korea
Konkuk University
Seoul South Korea
Sejong University
Seoul South Korea
Selçuk Üniversitesi
Selçuklu Turkey
University of Nyíregyháza
Nyíregyháza Hungary
Metrikler
6
Atıf
9
Yazar
7
Anahtar Kelime