Scopus
🔓 Açık Erişim
Ecdysteroids as Potent Enzyme Inhibitors and Verification of Their Activity Using In Vitro and In Silico Docking Studies
Life · Haziran 2022
Makale Bilgileri
DergiLife
Yayın TarihiHaziran 2022
Cilt / Sayfa12
Scopus ID2-s2.0-85131764811
Erişim🔓 Açık Erişim
Özet
Ecdysteroids represent arthropods’ steroidal hormones, and they exist in about 5–6% of plant species. In this study, the enzyme inhibitory activity of 20 ecdysteroids was assessed for the first time via determining their inhibition versus acetylcholinesterase, butyrylcholinesterase, tyrosinase, as well as α‐amylase enzymes. Furthermore, 20‐Hydroxyecdysone‐2,3,22‐tri‐O‐acetate (4) showed the highest inhibition of acetylcholinesterase and butyrylcholinesterase with values of 5.56 and 4.76 mg GALAE/g, respectively. All ecdysteroids displayed tyrosinase inhibitory effects, whereas the most potent was viticosterone E (7) with 78.88 mg KAE/g. Most ecdysteroids had similar amylase inhibitory properties; meanwhile, the best α‐amylase inhibitory potential was observed with viticosterone E‐diacetonide (18) (0.35 mmol ACAE/g). Most of the tested compounds showed tyrosinase inhibitory potential; therefore, they were exposed to molecular docking evaluation using the tyrosinase enzyme. Viticosterone E (7) showed the best ranking score with a docking score of −5.716 Kcal/mol and made three separate H‐bonds with Gly281, Asn81, and His85. From ADMET/TOPKAT in silico evaluation, it was obvious that most of the compounds displayed reasonable pharmacodynamic and pharmacokinetic properties; however, their toxicity should be carefully monitored by adjusting their doses while investigating their activity after incorporation into dosage forms. Principal component analysis (PCA) based upon the in vitro and in silico data was carried out to visualize the differences between the tested compounds better. PCA score plot successfully classifies the compounds into four main clusters that, in turn, reflects the similarities and differences among the clustered compounds with respect to their biological, pharmacokinetic, and pharmaco-dynamic properties that are mainly influenced by the similarity in the chemical structure. Thus, ecdysteroids can act as effective drug entities for alleviating several disorders owing to their enzyme inhibitory potential.
Yazarlar (9)
1
Nilufar Z. Mamadalieva
ORCID: 0000-0003-1756-3638
2
Hidayat Hussain
3
Adriano Mollica
4
Gokhan Zengin
ORCID: 0000-0001-6548-7823
5
Rano Mamadalieva
6
Sameh S. Elhady
ORCID: 0000-0003-3799-0581
7
Sana A. Fadil
8
Mohamed L. Ashour
ORCID: 0000-0002-9270-6267
9
Fadia S. Youssef
Anahtar Kelimeler
ADMET
chemometrics
cholinesterase
drug discovery
ecdysteroids
health care
molecular docking
tyrosinase
α‐amylase
Kurumlar
Academy of Sciences of the Republic of Uzbekistan
Tashkent Uzbekistan
Batterjee Medical College
Jeddah Saudi Arabia
Faculty of Pharmacy - Ain Shams University
Cairo Egypt
King Abdulaziz University
Jeddah Saudi Arabia
Kokand State Pedagogical Institute
Kokand Uzbekistan
Leibniz Institut fur Pflanzenbiochemie
Halle Germany
Selçuk Üniversitesi
Selçuklu Turkey
University of G. d'Annunzio Chieti and Pescara
Chieti Italy
Metrikler
2
Atıf
9
Yazar
9
Anahtar Kelime