Scopus
🔓 Açık Erişim
Antitumor Effect of Guatteria olivacea R. E. Fr. (Annonaceae) Leaf Essential Oil in Liver Cancer
Molecules · Temmuz 2022
Makale Bilgileri
DergiMolecules
Yayın TarihiTemmuz 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85135607136
Erişim🔓 Açık Erişim
Özet
Guatteria olivacea R. E. Fries (synonym Guatteria punctata (Aubl.) R.A. Howard) is a tree of 10–27 m tall popularly known as “envira-bobó”, “envira-fofa”, “envireira”, “embira”, “embira-branca”, “embira-preta”, envira-branca”, and “envira-preta”, which can be found in the Brazilian Amazon biome. In this study, we evaluated the cytotoxic and antitumor effects of the essential oil (EO) obtained from the leaves of G. olivacea against liver cancer using HepG2 cells as a model. EO was obtained using a hydrodistillation Clevenger-type apparatus and was qualitatively and quantitatively characterized using GC–MS and GC–FID, respectively. The alamar blue assay was used to assess the cytotoxic potential of EO in a panel of human cancer cell lines and human non-cancerous cells. In HepG2 cells treated with EO, YO-PRO-1/propidium iodide staining, cell cycle distribution, and reactive oxygen species (ROS) were examined. In C.B-17 SCID mice with HepG2 cell xenografts, the efficacy of the EO (20 and 40 mg/kg) was tested in vivo. GC–MS and GC–FID analyses showed germacrene D (17.65%), 1-epi-cubenol (13.21%), caryophyllene oxide (12.03%), spathulenol (11.26%), (E)-caryophyllene (7.26%), bicyclogermacrene (5.87%), and δ-elemene (4.95%) as the major constituents of G. olivacea leaf EO. In vitro cytotoxicity of EO was observed, including anti-liver cancer action with an IC50 value of 30.82 μg/mL for HepG2 cells. In HepG2 cells, EO treatment increased apoptotic cells and DNA fragmentation, without changes in ROS levels. Furthermore, the EO inhibited tumor mass in vivo by 32.8–57.9%. These findings suggest that G. olivacea leaf EO has anti-liver cancer potential.
Yazarlar (12)
1
Alexandre F.C. Galvão
2
Morgana de S. Araújo
3
Valdenizia R. Silva
4
Luciano de S. Santos
5
Rosane B. Dias
6
Clarissa A.Gurgel Rocha
ORCID: 0000-0001-8922-2985
7
Milena B.P. Soares
ORCID: 0000-0001-7549-2992
8
Felipe M.A.da Silva
ORCID: 0000-0002-1809-1372
9
Hector H.F. Koolen
ORCID: 0000-0002-0181-348X
10
Gokhan Zengin
ORCID: 0000-0001-6548-7823
11
Emmanoel V. Costa
ORCID: 0000-0002-0153-822X
12
Daniel P. Bezerra
ORCID: 0000-0002-6774-2063
Anahtar Kelimeler
antitumor
essential oil
Guatteria olivacea
liver cancer
Kurumlar
Amazonas State University (UEA)
Manaus Brazil
Fundacao Oswaldo Cruz
Rio de Janeiro Brazil
Selçuk Üniversitesi
Selçuklu Turkey
SENAI CIMATEC
Salvador Brazil
Universidade Federal da Bahia
Salvador Brazil
Universidade Federal do Amazonas
Manaus Brazil
Metrikler
4
Atıf
12
Yazar
4
Anahtar Kelime