Scopus
🔓 Açık Erişim YÖKSİS Eşleşti
Which Extraction Solvents and Methods Are More Effective in Terms of Chemical Composition and Biological Activity of Alcea fasciculiflora from Turkey?
Molecules · Ağustos 2022
YÖKSİS Kayıtları
Which Extraction Solvents and Methods Are More Effective in Terms of Chemical Composition and Biological Activity of Alcea fasciculiflora from Turkey?
Molecules · 2022 SCI-Expanded
PROFESÖR GÖKHAN ZENGİN →
Makale Bilgileri
DergiMolecules
Yayın TarihiAğustos 2022
Cilt / Sayfa27
Scopus ID2-s2.0-85136342858
Erişim🔓 Açık Erişim
Özet
The bioactive content, antioxidant properties, and enzyme inhibition properties of extracts of Alcea fasciculiflora from Turkey prepared with different solvents (water, methanol, ethyl acetate) and extraction methods (maceration, soxhlet, homogenizer assisted extraction, and ultrasound assisted extraction) were examined in this study. UHPLC-HRMS analysis detected or annotated a total of 50 compounds in A. fasciculiflora extracts, including 18 hydroxybenzoic and hydroxycinnamic acids, 7 Hexaric acids, 7 Coumarins, 15 Flavonoids, and 3 hydroxycinnamic acid amides. The extracts had phenolic and flavonoid levels ranging from 14.25 to 24.87 mg GAE/g and 1.68 to 25.26 mg RE/g, respectively, in the analysis. Both DPPH and ABTS tests revealed radical scavenging capabilities (between 2.63 and 35.33 mg TE/g and between 13.46 and 76.27 mg TE/g, respectively). The extracts had reducing properties (CUPRAC: 40.38–78 TE/g and FRAP: 17.51–42.58 TE/g). The extracts showed metal chelating activity (18.28–46.71 mg EDTAE/g) as well as total antioxidant capacity (phosphomolybdenum test) (0.90–2.12 mmol TE/g). DPPH, ABTS, FRAP, and metal chelating tests indicated the water extracts to be the best antioxidants, while the ethyl acetate extracts had the highest overall antioxidant capacity regardless of the extraction technique. Furthermore, anti-acetylcholinesterase activity was identified in all extracts (0.17–2.80 mg GALAE/g). The water extracts and the ultrasound-assisted ethyl acetate extract were inert against butyrylcholinesterase, but the other extracts showed anti-butyrylcholinesterase activity (1.17–5.80 mg GALAE/g). Tyrosine inhibitory action was identified in all extracts (1.79–58.93 mg KAE/g), with the most effective methanolic extracts. Only the ethyl acetate and methanolic extracts produced by maceration and homogenizer aided extraction showed glucosidase inhibition (0.11–1.11 mmol ACAE/g). These findings showed the overall bioactivity of the different extracts of A. fasciculiflora and provided an overview of the combination of solvent type and extraction method that could yield bioactive profile and pharmacological properties of interest and hence, could be a useful reference for future studies on this species.
Yazarlar (12)
1
Refiye Beyza Ozturk
2
Gokhan Zengin
ORCID: 0000-0001-6548-7823
3
Kouadio Ibrahime Sinan
4
Domenico Montesano
ORCID: 0000-0002-8857-2091
5
Dimitrina Zheleva-Dimitrova
ORCID: 0000-0002-1952-9903
6
Reneta Gevrenova
ORCID: 0000-0002-1254-2419
7
Abdullahi Ibrahim Uba
8
Ugur Cakilcioglu
9
Alevcan Kaplan
10
Sharmeen Jugreet
11
Stefano Dall'Acqua
12
Mohamad Fawzi Mahomoodally
Anahtar Kelimeler
Alcea fasciculiflora
antioxidant
enzyme inhibition
flavonoid
phenolic
solvents/extraction methods
Kurumlar
Batman University
Batman Turkey
Kadir Has Üniversitesi
Istanbul Turkey
Medical University of Sofia
Sofia Bulgaria
Munzur University
Tunceli Turkey
Selçuk Üniversitesi
Selçuklu Turkey
Università degli Studi di Napoli Federico II
Naples Italy
Università degli Studi di Padova
Padua Italy
University of Mauritius
Reduit Mauritius
Metrikler
9
Atıf
12
Yazar
6
Anahtar Kelime